丝胶抗氧化肽对H_2O_2诱导人脐静脉内皮细胞(ECV-304)损伤的保护作用  

Protection Effect of Antioxidant Peptides Derived from Silk Sericin on ECV-304 Cell Damage Induced by H_2O_2

作　　者：范金波[1] 周素珍[1] 郑立红[2] 吕长鑫[1] 冯叙桥[1] 任发政[3] 

机构地区：[1]渤海大学化学化工与食品安全学院,辽宁锦州121013 [2]河北科技师范学院食品工程系,河北昌黎066000 [3]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《中国食品学报》2014年第8期47-53,共7页Journal of Chinese Institute Of Food Science and Technology

摘　　要：通过H2O2诱导人脐静脉内皮细胞(ECV-304)损伤,建立细胞氧化损伤模型。研究丝胶抗氧化肽段SP2、SP3低、中、高质量浓度(50,100,200μg/mL)对细胞免受损伤的保护作用。研究结果表明:SP2和SP3(100,200μg/mL)处理组显著提高了细胞中总抗氧化能力(P<0.05),SOD,CAT和GSH-Px酶水平(P<0.05)以及细胞的NO水平(P<0.05);显著降低了细胞中MDA水平(P<0.05)。SP2、SP3肽段对H2O2诱导人脐静脉内皮细胞(ECV-304)的损伤有很好的保护作用,存在剂量依赖关系。本研究为开发丝胶抗氧化肽功能产品提供了理论依据,为合理开发和利用丝胶提供了新的途径。The model of ECV-304 cell damage induced by H2O2 was established. The effect on inhibiting ECV-304 cell damage of three dose treatment groups （50, 100, 200 μg/mL） of SP2 and SP3 were explorered. The results indicat- ed that treatment groups （100, 200 μg/mL ） of SP2 and SP3 increased activities of SOD, CAT, GSH-Px of cell （P〈0.05） and the level of NO （P〈0.05） and T-AOC of cell （P〈0.05）, decreased the level of MDA （P〈0.05）. The results indi cated that peptides SP2 and SP3 can protect ECV-304 cell damage induced by H2O2 and the effect depends on dose. The work will be a base for development of antioxidative functional food production and provide a method for potential exploitation and utilization of silk sericin.

关 键 词：丝胶 多肽 H2O2 细胞 保护作用 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]