假蒟地上部分和地下部分挥发性成分的GC-MS分析  

Analysis of the Volatility Constituents from the Ground Part and Underground Part of Piper sarmentosum by GC-MS

作　　者：刘雯露 何俏明 覃洁萍[1] 蔡毅[1] 洪江[1] 奉艳花 王淼[1] 

机构地区：[1]广西中医药大学,南宁530001

出　　处：《中国实验方剂学杂志》2014年第18期73-76,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西卫生厅资助项目(K428);广西自然科学基金创新研究团队项目(2011GXNSFF018006)

摘　　要：目的:分析假蒟地上部分和地下部分化学成分的差异,为其临床应用及研究开发提供参考。方法:采用石油醚和二氯甲烷梯度回流提取法,提取假蒟不同部位的化学成分,采用GC-MS法对其挥发性成分进行分析。结果:从假蒟地上部分共鉴定出36个成分,地下部分共鉴定出32个成分。共有成分有氢化肉桂酸、α-荜澄茄油烯、β-石竹烯、葎草烯、β-细辛脑、α-细辛脑、异榄香素等20种成分,含量分别占地上部分和地下部分总成分含量的85%以上和55%以上,其中含量最高的为α-细辛脑,均占不同部位化学成分总含量的30%以上。地上部分特有成分有白菖油萜、α-蛇床烯、d-杜松萜烯、β-桉叶醇、顺式-橙花叔醇、5-十八烷烯、角鲨烯等16种成分;地下部分特有成分有(1R)-(+)-α蒎烯、β-蒎烯、3-苯基丙酰胺等12种成分。结论:假蒟地上部分和地下部分挥发性成分虽大部分相同,但含量存在明显差异;两部分均含不同特有成分,可能与其不同药用性质相关。Objective：The aim of this study was to analyze the volatile constituents from the aerial part and the underground part of Piper sarmentosum,toprovide a basis for its development and clinical application.Method：The refluxing method was used to extract the chemical constituents from the root and the aerial part of P.sarmentosum with petroleum ether and dichloromethane.The components were separated and identified by GC-MS.Result：Thirty-six components in aerial part and 32 components in underground part were identified.Both the underground part and aerial part contained 20 common constituents,such as hydrocinnamic acid,α-cubebene,humulene,β-caryophyllene,β-asarone,α-asarone,isoelemicin and so on,which accounted for more than 85％ and 55％ of the total content of the aerial part and underground part,respectively.And α-asarpneshowed the highest content in each part,which is more than 30％.The aerial part contained 16 specific componentssuch ascalarene,α-selinene,d-cadinene,β-eudesmol,cis-nerolidol,5-octadecene,squalene and so on; whilethe underground part contained 12 specific components such as （1R） （＋）-α-pinene,β-pinene,3-phenylpropanamide and so on.Conclusion：Most of the volatile constituents from the aerial part and underground part of P.sarmentosumare identical,but their contents are obviously different.Andthere are specific constituents in both two parts,which might relate to theirdifferent medical usage.

关 键 词：假蒟 挥发性成分 气相色谱-质谱 地上部分 地下部分 

分 类 号：R284.1[医药卫生—中药学]