一种基于水解过程的多糖快速鉴别方法  

A hydrolysis process based rapid identification approach for polysaccharides

作　　者：马耀宏[1] 孟庆军[1] 杨艳[1] 杨俊慧[1] 史建国[1] 张利群[1] 郑岚[2] 刘伟霞[3] 

机构地区：[1]山东省科学院生物研究所,山东省生物传感器重点实验室,山东济南250014 [2]山东农业大学,山东泰安271018 [3]山东省计算中心,山东济南250014

出　　处：《山东科学》2014年第4期12-16,共5页Shandong Science

基　　金：国家高技术研究发展计划(863计划)(2012AA021201);山东省自主创新重大科技专项(20122501)

摘　　要：应用生物传感器记录树舌、灵芝和虫草多糖水解过程的还原糖(还原性末端数)和葡萄糖含量的实时变化,研究水溶性多糖水解过程中还原糖(还原性末端数)和葡萄糖的水解过程变化规律。研究表明,同一种多糖水解图谱稳定,不同种多糖图谱特异性明显,通过水解过程图谱的比对分析,可以实现对树舌、灵芝和虫草多糖的快速鉴别。We employed biosensors to record real-time variations of reducing sugar and glucose contents in hydrolysis process of Ganoderma applanatum Pat,Ganoderma lucidum and Cordyceps polysaccharide.We further investigated the variation law of the hydrolysis process of reducing sugar and glucose in hydrolysis process of water-soluble polysaccharide.Results show that hydrolysis process map is stable for the same kind of polysaccharide,but the specificity of hydrolysis process map is significant for different kinds of polysaccharide.Rapid identification of Ganoderma applanatum Pat,Ganoderma lucidum,and Cordyceps polysaccharide can be implemented by the comparison analysis of hydrolysis process map.

关 键 词：多糖 水解过程 葡萄糖 还原糖 

分 类 号：Q81[生物学—生物工程] TP212.3[自动化与计算机技术—检测技术与自动化装置]