载银氨基硅胶柱分离EPA和DHA的研究  

Separation of EPA and DHA by Ag^+ modified amino silica gel column

作　　者：邢飞[1] 朱靖博[1,2] 丁燕[1,2] 寇自农[2,3] 

机构地区：[1]大连工业大学食品学院,辽宁大连116034 [2]大连工业大学植物资源化学与应用研究所,辽宁大连116034 [3]大连工业大学现代教育技术部,辽宁大连116034

出　　处：《中国油脂》2014年第9期71-73,共3页China Oils and Fats

基　　金：辽宁省高等学校重大科技平台项目(辽教发[2011]191号)

摘　　要：利用负载银离子的氨基硅胶为固定相,制备4.6 mm×250 mm载银氨基硅胶柱,系统评价EPA和DHA在正相体系中的色谱保留行为,考察了载银氨基硅胶的粒径和流动相对EPA和DHA分离的影响。结果表明:5μm粒径的载银氨基硅胶比10μm粒径的分离度好;随着流动相中正己烷比例的增大,EPA和DHA分离度增大;当选用5μm载银氨基硅胶、流动相为正己烷-异丁醇(体积比2 000∶1)、流速1 mL/min、紫外检测波长210 nm、进样量5μL时,EPA和DHA的分离度最佳,分离度为2.03。该方法简单、快速、重现性好,可用于EPA和DHA的分离。The analysis column （4. 6 mm × 250 mm） for the separation of EPA and DHA was prepared with Ag＋ modified amino silica gel as stationary phase. A systematic evaluation on retention behavior of EPA and DHA in normal phase chromatography was performed. The influences of Ag＋ modified amino silica gel with different pore sizes and mobile phase on the separation of EPA and DHA were researched. The results showed that the separation degree of 5 μm Ag＋ modified amino silica gel was better than that of 10 μm Ag＋ modified amino silica gel;the separation degree of EPA and DHA increased with the pro-portion of n-hexane in mobile phase increasing;when the pore size of Ag＋ modified amino silica gel was 5 μm, the volume ratio of n-hexane to isobutanol was 2 000∶1, flow rate was 1 mL/min, ultraviolet de-tection wavelength was 210 nm and the injection volume was 5 μL, the separation degree of EPA and DHA was the best and reached 2. 03. The method had the items of simpleness, rapidness and high repro-ducibility, which was suitable for the separation of EPA and DHA.

关 键 词：EPA DHA 正相色谱 载银氨基硅胶 

分 类 号：TQ645.6[化学工程—精细化工]