抚顺盆地西露天组油页岩微生物碳代谢多样性分析  

Carbon-source metabolic diversity of oil shale microorganisms in Fushun Basin Western Open Group

作　　者：蒋绍妍 王文星[2] 薛向欣[1] 

机构地区：[1]东北大学材料与冶金学院,辽宁沈阳110819 [2]东北大学生命科学与健康学院,辽宁沈阳110819

出　　处：《中国环境科学》2014年第9期2321-2327,共7页China Environmental Science

基　　金：国家自然科学基金资助项目(51204055);中国博士后科学基金资助项目(20100481205);中央高校基本科研业务费专项资金资助项目(N130420002)

摘　　要：为探讨油页岩微生物的碳代谢功能多样性,以抚顺盆地西露天组油页岩为研究对象,利用Biolog-GN2和GP2微孔板检测法,在连续7d的培养期内,测定油页岩本源微生物群落水平生理图谱(CLPP),并根据化学基团的不同,分析微生物对不同类型碳源的代谢能力和代谢差异.结果显示,微生物对不同类型碳源的代谢程度和代谢速率存在明显的差异.革兰氏阴性和阳性细菌群落均对氨基类碳源的利用较好,其次是羧酸类以及糖和糖衍生物,而对磷酸糖利用较差.连续多点测算Shannon-Wiener,Simpson和McIntosh多样性指数,分析表明微生物群落碳代谢功能多样性总体较高,但是随着培养时间的增加,多样性略有降低.Functional diversity of carbon-source metabolism of oil shale microorganisms in Fushun Basin Western Open Group was investigated by Biolog-GN2 and GP2 microplates method. The microbial community level physiological profiles （CLPP） were determined during 7 consecutive days. According to different chemical groups, the metabolic capacity and differences of microbial communities on different carbon sources were analyzed. The results showed that the metabolic level and metabolic rate of microbial communities on different carbon sources were obviously different. For the Gram-negative or Gram-positive bacteria communities, the utilization of carbon sources with amino group was the highest, followed by carboxylic acid, sugar and its derivatives, and sugar phosphates was the lowest. The analyses of Shannon-Wiener, Simpson and McIntosh indexes through multi-point calculation indicated that the carbon metabolic diversity of microbial communities in oil shale was generally high, but the diversity slightly decreased with incubation time.

关 键 词：油页岩 功能多样性 BIOLOG 碳代谢 多样性指数 

分 类 号：X172[环境科学与工程—环境科学]