不同搅拌转速和通气量对桑黄深层发酵培养的影响  

Effects of Impellor Speed and Aeration Rate on Mycelial Biomass and Extracellular Polysaccharide Production,Reducing Sugar Consumption and Dissolved Oxygen Levels in Fermentor-grown Cultures of Phellinus baumii

作　　者：雷萍[1] 吴亚召[1] 张文隽[1] 陈旭[2] 吕德平[2] 安军民[3] 

机构地区：[1]陕西省微生物研究所,陕西西安710043 [2]中华全国供销合作总社昆明食用菌研究所,云南昆明6502213 [3]西安市第四医院,陕西西安710004

出　　处：《食用菌学报》2014年第2期57-62,共6页Acta Edulis Fungi

基　　金：陕西省科学院应用基础与产业化项目"药用真菌桑黄菌丝体产业化开发研究"(编号:2012K-17);西安市科技局技术转移促进工程(高校类)"生物医药新产品新技术开发--药用真菌桑黄发酵菌丝体的研究与开发"(编号:CXY1348-4)的部分研究内容

摘　　要：探讨在20L搅拌式发酵罐中,转速和通气量对桑黄菌丝生物量、胞外多糖产率、溶氧以及菌丝形态的影响。结果表明,实验范围内,转速为150r/min时,桑黄菌丝体生物量最大(12.65g/L),胞外多糖得率最高(2.99g/L),相对溶氧下降最快,菌球小球状、较紧密、丝状体比例小;通气量为1∶0.65vvm,桑黄菌丝体生物量最大(12.69g/L),胞外多糖得率最高(3.00g/L),相对溶氧下降最快,菌球大小均匀、紧密、生长良好。The effects of impellor speed and aeration rate on mycelial biomass and extracellular polysaccharide production,reducing sugar consumption and dissolved oxygen levels during growth of Phellinus baumii in a 20-L fermentor were determined.Highest biomass （12.65 g/L）and extracellular polysaccharide（2.99 g/L） yields,and the most rapid decrease in dissolved oxygen levels,were recorded when the impellor speed was set at 150 r/min.Mycelial pellets were globose and compact,and the proportion of hyphal filaments was low. Highest yields of mycelial biomass （12.69 g/L）and extracellular polysaccharide（3.0 g/L）,and the most rapid decrease in dissolved oxygen levels,were recorded when the aeration rate was set at 1∶0.65 vvm （air volume/culture volume/min）.At this setting,the fungal mycelium grew well and formed compact pellets of uniform size.

关 键 词：桑黄 深层发酵 搅拌转速 通气量 

分 类 号：S567.39[农业科学—中草药栽培]