细脚棒束孢E3菌株产N6-(2-羟乙基)腺苷的液体发酵条件优化  

Optimization of Selected Parameters Affecting N^6-(2-Hydroxyethyl)-adenosine Production by Isaria tenuipes Strain E3 Grown in Submerged Culture

作　　者：雷帮星[1] 何劲[2] 康冀川[1] 余信[1] 桂江伟 文庭池[1] 

机构地区：[1]贵州大学,贵州省生化工程中心,贵州贵阳550025 [2]贵阳学院,贵州贵阳550005

出　　处：《食用菌学报》2014年第2期63-66,共4页Acta Edulis Fungi

基　　金：贵州省社会发展科技攻关计划[编号:黔科合SY字(2008)3047];贵州省科技计划[编号:黔科合高G字(2010)4009];贵州省科学技术基金[编号:黔科合J字(2012)2102号]的部分研究内容

摘　　要：以N6-(2-羟乙基)腺苷[N6-(2-hydroxyethyl)-adenosine,HEA]的产量为评价指标,查氏培养基为基础培养基,分别筛选液体静置发酵的光照、温度、时间、培养基初始pH、装液量和接种量,结果表明:有利于细脚棒束孢(Isaria tenuipes)E3菌株产HEA的发酵条件为黑暗或荧光、黄光,培养温度为25~28℃,培养时间为110d,培养基的初始pH为6.0~7.0,装液量为50%,接种量为2.5%。Selected parameters （light source,temperature,pH,culture time,volume of culture medium, inoculum size）affecting N6-（2-hydroxyethyl）-adenosine （HEA）production by Isaria tenuipes,strain E3, grown in static submerged culture on Czapek medium have been optimized.Maximum N6-（2-hydroxyethyl）-adenosine production was recorded using fluorescent or yellow light sources,a growth temperature range of 25~28 ℃,a growth medium pH range of 6.0~7.0,a culture time of 110 days,a culture volume of 150 mL in a 300 mL flask,and a 2.5% inoculum size.

关 键 词：细脚棒束孢 N6-(2-羟乙基)腺苷 发酵 

分 类 号：S567.3[农业科学—中草药栽培]