以油莎豆为原料发酵生产威兰胶的研究  

Welan gum production from cyperus esculentus fermented by sphingomonas sp. ATCC 31555

作　　者：龙柯[1] 李响[1] 谢通慧[1] 张永奎[1] 刘文彬[1] 

机构地区：[1]四川大学化学工程学院制药与生物工程系,四川成都610065

出　　处：《化学工程师》2014年第8期1-5,共5页Chemical Engineer

基　　金：科技型中小企业技术创新资金项目(No.10C26215104937);四川省科技型中小企业早期科技创新省级扶持资金无偿补助项目;国家级大学生创新创业训练计划项目(No.201210610036)

摘　　要：为降低威兰胶生产成本,提高其产量,以油莎豆为原料,通过提取油脂、蛋白质和水解等处理,制得豆饼粕水解液作为底物,发酵生产威兰胶。结果表明:在不添加其他氮源或营养盐条件下,以水解液为底物生产威兰胶,产量为24.12g·L-1,较葡萄糖发酵组提高26.68%,威兰胶产品的剪切性能值为4.40。使用油莎豆粕水解液发酵生产威兰胶,发酵培养基中不需添加额外碳氮源,产量和剪切性能均优于利用葡萄糖发酵的结果。由此可见,油莎豆是生产威兰胶的一种优质原料。To lower the production cost of welan gum and raise the production,how to utilize the C.esculentus waste hydrolysate （CEWH）, which has been extracted its oil and part of the protein, as the substrate to ferment welan gum was investigated. Under the condition of using CEWH as substrate, and adding neither nitrogen source nor nutritive salt, the production of welan gum reached the maximum of 24.12g＆#183;L-1, which was 26.68% more than that of using glucose to ferment. The values of shear properties of welan gum also come to 4.40. When using the C. esculentus waste hydrolysate to ferment the welan gum, there is no need to add other carbon source nor nitrogen source in the fermentation medium and both the production as well as the sheer properties are better than the re-sults of using glucose to ferment. Thus, the C.esculentus is a cheap and high quality raw material to produce the welan gum.

关 键 词：廉价底物 油莎豆水解液 威兰胶 发酵 

分 类 号：Q93[生物学—微生物学]