虾青素对氧化应激诱导内皮祖细胞凋亡的保护作用  

Protective effect of astaxanthin on endothelial progenitor cell apoptosis induced by oxidative stress

作　　者：李培[1] 张琼宇[1] 曾乐平[2] 龚志刚[3,4] 

机构地区：[1]湖南永州职业技术学院医学院,湖南永州425006 [2]中南大学基础医学院人体解剖与神经生物学系,长沙410013 [3]南方医科大学研究生院,广州510515 [4]广州军区武汉总医院心内科,武汉430070

出　　处：《重庆医学》2014年第26期3464-3467,共4页Chongqing medicine

摘　　要：目的探讨虾青素对体外氧化应激诱导的人外周血内皮祖细胞凋亡的影响及机制。方法体外培养人外周血单核细胞源的内皮祖细胞,分为对照组、模型组(叔丁基过氧化氢100μmol/L)、虾青素+叔丁基过氧化氢组[虾青素0.10、1.00、10.00nmol/L预处理24h后,分别使用叔丁基过氧化氢溶液(100μmol/L)连续培养6h]。细胞存活率采用MTT法检测;细胞凋亡率采用DAPI染细胞检测;DCFH-DA法检测细胞内活性氧(reactive oxygen species,ROS)水平;JC-1法测定线粒体膜电位。结果与对照组比较,叔丁基过氧化氢(100μmol/L)能明显的造成内皮祖细胞的凋亡(P<0.05)。虾青素可降低叔丁基过氧化氢引起的内皮祖细胞凋亡,表现为细胞凋亡率减少(P<0.05),线粒体膜电位增加。结论虾青素对叔丁基过氧化氢诱导的内皮祖细胞凋亡具有保护作用,其机制可能与保护线粒体功能有关。Objective To investigate the effect of astaxanthin on the peripheral blood endothelial progenitor cells （EPCs） apop-tosis induced by oxidative stress in vitro and to explore its underlying mechanism .Methods Human peripheral blood EPCs were in vitro cultured and divided into the control group ,model group with 100 μmol/L tert-butyl hydroperoxide（tBHP） and the astaxan-thin plus tBHP group（with 0 .10 ,1 .00 ,10 .00 nmol/L astaxanthin pretreatment for 24 h ,then adding the final concentration of 100μmol/L tBHP for 6 h continuous culture） .The cell viability was measured by the MTT method .The level of reactive oxygen spe-cies （ROS） was determined by the DCFH-DA method .The changes of mitochondrial membrane potential（MMP） and the apoptosis ratio were detected by the JC-1 method and the DAPI method ,respectively .Results Compared with control group ,100μmol/L tB-HP could obviously caused the apoptosis of EPCs（P〈0 .05） ,while astaxanthin could decrease tBHP induced apoptosis ,which man-ifested by the decrease of the apoptosis ratio （P〈0 .05） and MMP increase .Conclusion Astaxanthin has the protective effect on the apoptosis of EPCs ,its mechanism may be related with the protection of the mitochondrial function .

关 键 词：氧化性应激 内皮祖细胞 虾毒素 细胞凋亡 膜电位 线粒体 

分 类 号：R363[医药卫生—病理学]