黄山石耳多糖的提取及其抗氧化性研究  

Optimal process of extraction and antioxidant activity of polysaccharide fromUmbilicaria esculenta (Miyoshi) Minks in Yellow Mountain

作　　者：姚玉飞[1] 张海林[1] 刘咏[1] 杜逸群[1] 张建波[1] 王军辉[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《合肥工业大学学报（自然科学版）》2014年第9期1132-1137,共6页Journal of Hefei University of Technology：Natural Science

基　　金：国家自然科学基金资助项目(31370371);安徽省国际科技合作计划资助项目(12030603020);国家级大学生创新训练资助项目(201310359064);江南大学食品胶体与生物技术教育部重点实验室开放课题研究基金资助项目(JDSJ2012-04)

摘　　要：文章研究了黄山石耳多糖的复合酶法辅助提取工艺及其抗氧化性。通过单因素和L9(33)正交实验确定复合酶的最佳添加量为每0.2g样品中添加240U纤维素酶、720U果胶酶和2 400U木瓜蛋白酶;通过单因素和L9(34)正交实验优化石耳多糖的提取工艺,得出最优条件,即提取温度为60℃,提取时间为1h,pH值为6和料液比1∶40,该工艺条件下多糖提取率为12.52%;通过单因素和L9(33)正交实验优化石耳多糖的醇沉工艺,得出最优醇沉时间为5h,醇沉温度为5℃,乙醇体积分数为80%,此时多糖最终提取率为10.52%。黄山石耳多糖的体外抗氧化性实验结果表明,石耳多糖具有较高的体外抗氧化活性,其对ABTS自由基、DPPH自由基和OH自由基的清除率分别达到82.95%、74.58%和70.87%。The optimal process of extraction with combined-enzyme method and antioxidant activity of polysac-charide from Umbilicaria esculenta in Yellow Mountain were investigated in this paper .Firstly ,the best ratio of cellulase ,pectinase and papain was determined by single factor and L 9 （33 ） orthogonal experiments ,the op-timum amount is adding 240 U cellulase ,720 U pectinase and 2 400 U papain per 0.2 g polysaccharide sam-ple .Then ,the extraction time ,extraction temperature ,pH value ,and material-water ratio of the optimal ex-traction of polysaccharide were considered by single factor and L 9 （34 ） orthogonal experiments ,the best ex-traction temperature was 60 ＆#176;C ,extraction time was 1 h ,pH value was 6 ,and material-solvent ratio was 1∶40（W/V） .The yield of polysaccharides reached to 12.52% after optimal process .Besides ,the alcohol pre-cipitation time ,alcohol precipitation temperature ,and ethanol concentration ratio of the optimal alcohol pre-cipitation of polysaccharide were considered by single factor and L 9 （33 ） orthogonal experiments and the results showed that the optimal alcohol precipitation temperature was 5 ＆#176;C ,alcohol precipitation time was 5 h ,and ethanol concentration ratio was 80% （V/V） .The polysaccharide yield proportion was 10.52% .Finally ,the in-vitro antioxidant experimental results indicated that the crude polysaccharide had good antioxidant activi-ties ,and the scavenging activities of ABTS free radical ,DPPH free radical and OH free radical approached 82.95% ,74.58% and 70.87% ,respectively .

关 键 词：石耳 多糖 复合酶法 工艺优化 抗氧化性 

分 类 号：TQ91[化学工程]