超声波辅助提取石阡苔茶多糖工艺的优化  

Optimizing the Polysaccharide Extraction Process from Shiqian Moss Tea by Ultrasonic-assisted Method

作　　者：陈仕学[1] 王岚[1] 代鸣[1] 王一帆[1] 杨芳[1] 

机构地区：[1]铜仁学院生物科学与化学系/梵净山特色动植物资源重点实验室/梵净山野生动植物资源保护与利用研究中心,贵州铜仁554300

出　　处：《湖北农业科学》2014年第15期3609-3612,共4页Hubei Agricultural Sciences

基　　金：铜仁市科技计划项目[铜市科研(2012)63号-23];贵州省教育厅特色实验室建设项目[黔教合KY(2011)232];贵州省高等学校重点支持学科项目[黔教合重点支持学科字(2011)232]

摘　　要：为了研究超声波辅助提取石阡苔茶[Camellia sinensis(L.)O.Ktze.]多糖的最佳提取工艺,采用单因素和正交试验进行研究,得到了提取石阡苔茶多糖的最佳工艺条件为乙醇体积分数40%,超声功率100W,浸提时间30 min,料液比1∶40(g∶mL)。此条件下多糖的平均提取率为4.92%,RSD为0.42%。可见,超声波辅助提取法缩短了浸提时间,节约了材料,提高了石阡苔茶多糖的提取率。The optimal extraction process of polysaccharide from Shiqian Moss Tea[Camellia sinensis （L.） O. Ktze.l was stud- ied with the ultrasonic-assisted method. The single factor and the orthogonal test were adopted. The results showed that its optimal conditions were 40% ethanol, 100 W ultrasonic power, 30 minutes ultrasonic digestion time and 1：40 （g：mL） solid- liquid ratio. Under these conditions,the avarage extraction rate of the polysaccharide was 4.92% and that of RSD was 0.42%. The ultrasonic-assisted method can greatly shorten the extraction time, save the materials and enhance the extraction rate of Shiqian Moss Tea＇s polysaccharides.

关 键 词：苔茶[Camellia SINENSIS (L.)O.Ktze.] 多糖 超声波辅助法 提取率 工艺优化 

分 类 号：TQ461[化学工程—制药化工]