基于现代电子舌技术的传统苦味中药黄连的苦味物质基础研究  

Research on bitter components from Coptis chinensis based on electronic tongue

作　　者：梁晓光[1] 吴飞[1,2] 王优杰[1,2] 富臻 王源[1] 冯怡[1,2] 梁爽[1] 

机构地区：[1]上海中医药大学中药现代制剂技术教育部工程研究中心,上海201203 [2]上海张江中药现代制剂技术工程研究中心,上海201203

出　　处：《中国中药杂志》2014年第17期3326-3329,共4页China Journal of Chinese Materia Medica

基　　金：上海市教委预算内科研项目(2012JW28)

摘　　要：对代表性苦味中药黄连进行色谱分离,得到小檗碱(berberine),非洲防己碱(columbamine),groenlandicine,药根碱(jatrorrhizine),木兰花碱(magnoflorine),corydaldine和阿魏酸甲酯(ferulic acid methylester)7种化合物。对其中的6种生物碱进行电子舌苦度评价及抑菌活性研究,并对其结果进行相关性评价。结果显示,具有明显苦度的小檗碱,非洲防己碱,groenlandicine,药根碱同时对大肠杆菌,金黄色葡萄球菌有一定的抑菌活性,而苦度较小的木兰花碱,corydaldine则没有抑菌活性;苦味化合物苦度与其对大肠杆菌和金黄色葡萄球菌抑菌活性的相关性系数分别为0.983,0.911。因此可以得出结论,黄连中苦味化合物的苦度与抑菌活性呈显著正相关,并进一步证实苦味化合物应为苦味药中的主要药效物质基础。Isolated alkaloids from Coptis chinensis Franch. The compounds were identified as berberine, columbamine, groenlandicine, jatrorrhizine, magnoflorine, corydaldine and ferulic acid methylester. Then measured their bitter degree based on the electronic tongue and evaluated the antibacterial. The results based on the Electronic Tongue showed that berberine, columbamine, groenlandicine and jatrorrhizine have higher bitter degree than magnoflorine and corydaldine. And they also appeared better antibacterial activity on E. coli and S. aureus. The correlation coefficients between bitter degree and the two bacteria antibacterial activity were O. 983 and O. 911. So there was close relationship between the bitter degree and antibacterial activity of bitter components. Thus, it is confirmed further that bitter components are the material foundation of medicinal effectiveness of bitter herbs.

关 键 词：苦味药 苦味物质 药效物质 黄连 电子舌 抑菌 

分 类 号：R285.1[医药卫生—中药学]