HPLC法检测抗氧化包装膜中槲皮素的保留率  

Determination of Quercetin Retention in Antioxidant Food Package Film by High Performance Liquid Chromatography

作　　者：韩甜甜 卢立新 

机构地区：[1]江南大学,无锡214122 [2]江苏省食品先进制造装备技术重点实验室,无锡214122

出　　处：《包装工程》2014年第19期13-17,28,共6页Packaging Engineering

基　　金：国家科技支撑计划(2011BAD24B01);江苏省高校科研成果产业化推进工程项目(JHB2012-25)

摘　　要：目的 建立一种快速准确地测定在含有槲皮素的食品抗氧化包装膜中槲皮素保留率的测定方法。方法 在温度为65℃的纯乙醇溶液中,将含有槲皮素的食品薄膜抽提10 h,重复1次抽提过程,得到含槲皮素的抽提液,采用高效液相色谱法测定抽提液中槲皮素的含量。结果 槲皮素回归方程为y=643.65x＋0.0572（R2=0.9995,n=6）,在槲皮素的质量浓度为1.6～280μg/mL时线性良好,平均加样回收率为99.69%（RSD=1.77%）。结论 该检测方法出峰快,能有效改善峰形,避免拖尾峰的出现。该方法简便快速、易操作、准确可靠,可用于检测抗氧化食品包装膜中槲皮素的保留率。Objective To establish a method for the determination of quercetin retention in antioxidant food package film by HPLC. Methods Quercetin was extracted in ethanoI at 65 ℃ for 10 h and the extraction was repeated once. Results The detection method was fast, and could effectively improve the peak shape as well as avoid peak tailing. The linear regression equation for quercetin was y=643.65x＋0.0572 （R2=0.9995, n=6） and the linear range was 1.6-280μg/mL. The average recovery was 99.69% （RSD=l.77%） for quercetin. Conclusion This was a simple, rapid and accurate method to detect the quercetin retention in the antioxidant food package film.

关 键 词：槲皮素 高效液相色谱 食品包装膜 抽提 含量测定 

分 类 号：TS206[轻工技术与工程—食品科学]