农产品中赭曲霉毒素A免疫层析试纸条快速检测技术研究  

Development of an immunochromatographic assay for ochratoxin A in agro-products

作　　者：李鑫[1,2,3] 李培武[1,2,3,4,5] 张奇[1,2,3] 张文[1,2,5] 丁小霞[1,2,4] 唐晓倩[1,3,5] 

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062 [2]农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [3]农业部生物毒素检测重点实验室,湖北武汉430062 [4]农业部油料产品质量安全风险评估实验室(武汉),湖北武汉430062 [5]农业部油料及制品质量监督检验测试中心,湖北武汉430062

出　　处：《中国油料作物学报》2014年第5期648-652,共5页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31101299);国家科技支撑计划(2012BAB19B09);公益性农业行业专项(201203094)

摘　　要：基于胶体金免疫层析原理建立了一种快速检测赭曲霉毒素A(ochrotaxin A,OTA)的胶体金试纸条的研制方法,包括胶体金的制备、金标记探针的制备、试纸条各条件参数的测试和优化等。试纸条检测OTA的肉眼可视检测限为0.25ng/mL,检测时间为10min,试纸条能特异性识别OTA而不与赭曲霉毒素B及其他真菌毒素发生交叉反应,使用简单、方便、成本低,重复性好,保质期长,尤其适用于农产品中OTA的现场快速筛查。This study developed a colloidal gold immunochromatographic assay method for rapid detection of ochratoxin A( OTA),based on the theory of colloidal gold immunochromatography. Production of colloidal gold and labeled probe were evaluated,and parameters of strip testwere optimized. The sensitivity of the strip for OTA was 0. 25 ng /mL performed in 10 min. The strip could specially recognize OTA and did not react with ochratoxin B and other mycotoxins. This method was simple,convenient and low- cost,could be easily repeated and had long shelf time. It was particularly suitable for on- site screening detection of OTA in agricultural products.

关 键 词：赭曲霉毒素A 胶体金 免疫层析 试纸条 快速检测 

分 类 号：TS207[轻工技术与工程—食品科学]