复凝聚法制备球孢白僵菌微胶囊  

Study on the Preparation of Beauveria bassiana Microcapsules by Complex Coacervation

作　　者：李姗[1] 王志英[1] 

机构地区：[1]东北林业大学,黑龙江哈尔滨150040

出　　处：《安徽农业科学》2014年第29期10179-10182,10215,共5页Journal of Anhui Agricultural Sciences

基　　金："十二五"农村领域国家科技计划项目(2011BAD37B01-4);黑龙江省科技计划项目(GA09B203-3)

摘　　要：[目的]研究复凝聚法制备以明胶和阿拉伯胶为壁材的球孢白僵菌(Beauveria bassiana)微胶囊的工艺。[方法]通过单因素试验研究了明胶浓度、阿拉伯胶浓度、芯壁比、pH、反应温度和转速对微胶囊包埋效率的影响,再通过正交优化试验确定最佳制备条件;利用光学显微镜、扫描电子显微镜和粒径测微仪对球孢白僵菌微胶囊形态及粒径分布进行表征。[结果]复凝聚法制备球孢白僵菌微胶囊最佳工艺条件为明胶浓度1.5%、阿拉伯胶浓度0.5%、芯壁比1.0∶1.5、复凝聚反应pH 3.8、搅拌速度450 r/min,在该条件下可制得包埋率达78.32%、粒径分布均匀且平均粒径在44.16μm左右、流动性好的球型固体微胶囊。[结论]该研究制备的微胶囊符合实际应用需求,为真菌杀虫剂的研制提供了理论依据。The aim was to study the preparation technology of Beauveria bassiana microcapsule by complex coacervation method from gelatin and Arabic gum as wall material. [Method]The effect of processing parameters which included the different wall material concentration,the ratio between the wall material,the ratio of core to skin,pH,reaction temperature and the stirring speed on the efficiency of microencapsulated was evaluated by single factor experiments. The optimal preparation process condition was obtained by applying orthogonal optimization experiments. The microcapsules were characterized by optical microscope,scanning electron microscope and laser particle size analyzer. [Result]The optimum condition was fellows： gelatin concentration was 1. 5%,arabic gum concentration was 0. 5%,the ratio of core to skin was 1. 0∶1. 5,the complex coacervation reaction pH value was 3. 8,and stirring speed was 450 r /min. The microencapsulation efficiency can reach 78. 32%. The spherical solid microcapsules,which was liquidity,with even distribution of particle size and average diameter of about 44. 16 μm. [Conclusion]The microcapsules can fully meet the needs of practical applications,and the research provides theoretical basis for the preparation of pesticides.

关 键 词：球孢白僵菌 明胶 阿拉伯胶 复凝聚法 微胶囊 

分 类 号：S482.3[农业科学—农药学]