HPLC-ELSD法测定保健食品中低聚木糖  

Determination of Xylo-Oligosaccharides in Health Foods by HPLC-ELSD

作　　者：毕晓黎 程青云[2] 彭丽诗[2] 罗文汇 李养学 

机构地区：[1]广东省中医药工程技术研究院,广东广州510095 [2]广州中医药大学,广东广州510405

出　　处：《辽宁中医药大学学报》2014年第10期7-8,共2页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：广州市科技计划民生科技重大专项项目(201300000062)

摘　　要：目的：建立高效液相色谱-蒸发光散射检测器（HPLC-ELSD）测定保健食品中低聚木糖（以木糖计）的方法。方法：样品经醇沉去除杂质,后用硫酸在沸水浴中将低聚木糖水解为木糖,以乙腈-水（80∶20,v/v）为流动相,糖分析柱（4.6 mm×250 mm,5μm）为固定相,通过蒸发光散射检测器的检测,同一样品水解前后木糖含量的差值即为样品中低聚木糖的含量。结果：低聚木糖在0.6796～5.097μg范围内呈良好的线性关系,r=1,平均加样回收率为101.66%,RSD为1.39%（n=9）。结论：该方法快速、简便、灵敏、重现性好,可作为测定保健食品中低聚木糖的方法。Objective： To develop an HPLC-ELSD method for the determination of xylooligosaccharides in health foods. Method： The sample was removed impurity by alcohol precipitation method and the xylo-oligosaccharides was hydrolyzed into xylogyranose with sulfuric acid solution in boiling water bath, and then sapareted on a carbohydrate column with a mixture of acetonitrile-water （ 80 ： 20, v/v ） as mobile phase. Detected by ELSD detector, the xylo-oligosaccharides content was the D-value of xylogyranose content before and after the hydrolyzation. Result ： The linear ranges of ferulic acid was 0.6796-5.097 μg. The average recovery rate for xylo-oligosaccharides was 101.66%, and RSD was 1.39% （ n=9 ）. Conclusion ： The developed method is rapid, simple, sensitive and replicahle, which can be used for the determination of xylo-oligosaccharid in health foods.

关 键 词：高效液相色谱 蒸发光散射检测器 低聚木糖 木糖 

分 类 号：R927.2[医药卫生—药学]