肺癌细胞RhoA、RhoB和RhoC的表达及意义  

Expression of RhoA, RhoB and RhoC in Lung Cancer Tissues and Their Clinical Significance

作　　者：狄松波 刘立荣[1] 曹奇峰[1] 莫巍[1] 王丹萍[1] 

机构地区：[1]浙江省台州市中西医结合医院,台州317523

出　　处：《浙江中西医结合杂志》2014年第9期761-764,I0002,共5页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省医药卫生科学研究基金资助项目(No.2007A196)

摘　　要：目的通过研究RhoA、RhoB和RhoC在肺癌组织及细胞系中表达水平的变化,探讨Rho亚家族与肺癌发生、发展及转移的关系。方法应用荧光定量RT-PCR法、Western blotting法研究四种肺癌细胞系RhoA、RhoB和RhoC mRNA和蛋白表达水平变化,免疫组织化学法检测45例人肺癌组织RhoA、RhoB和RhoC蛋白表达。结果四种肺癌细胞系RhoA和RhoB mRNA和蛋白表达水平显著高于对照组(P均<0.05),肺癌细胞系组间比较,表达水平差异无统计学意义(P均>0.05),各组间RhoC mRNA和蛋白表达水平差异无统计学意义(P均>0.05)。RhoA、RhoB蛋白在人肺癌组织呈阳性表达,中分化鳞癌组阳性率与非中分化鳞癌组阳性率差异无统计学意义(P均>0.05),而RhoC全部呈阴性表达。结论 RhoA、RhoB在肺癌组织中异常高表达,提示Rho亚家族参与了肺癌的发生、分化和转移过程。To investigate changes of expressions of RhoA and RhoB and RhoC in lung cancer tissues to explore the relationship of Rho subfamily with development,migration,and metastasis of lung carcinoma. Methods The expressions of RhoA,RhoB and RhoC mRNA and protein in four different lung cancer cell lines （LTEP-a-2,SK-MES-1,NCI-H446,and NCI-H292） were detected by real-time quantitative reverse transcription polymerase chain reaction（QRT-PCR） and Western blotting, and were compared with MRC-5（control group）. The expressions of RhoA,RhoB and RhoC protein in 45 cases of lung cancer tissue by immunocytochemistry. Results The expressions of RhoA and RhoB mRNA and protein in lung cancer cell line LTEP-a-2,SK-MES-1,NCI-H446,and NCI-H292 were significantly higher than those in control group （all P〈0.05）,while no statistical difference was found among four lung cancer cell lines（all P〉0.05）. The expressions of RhoA and RhoB protein in human lung cancer tissue were positive,but the levels of them were not statistically different between moderately differentiated squamous carcinoma group and non-moderately differentiated squamous carcinoma group （all P〉0.05）. The expression of RhoC protein was negative. Conclusion The expressions of RhoA and RhoB were elevated in lung cancer,which indicates that Rho subfamily involve in the development,migration and metastasis of lung cancer.

关 键 词：肺癌 RHOA RHOB RHOC 

分 类 号：R734.2[医药卫生—肿瘤]