山葡萄‘双红’和‘双优’的试管快繁研究  

Rapid proliferation of Vitis amurensis ‘Shuanghong' and ‘Shuangyou'

作　　者：戴彩虹[1] 马绍英[1] 李胜[1] 苏李维 时振振 苏利荣[1] 胡翠珍[1] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2014年第4期63-68,72,共7页Journal of Gansu Agricultural University

基　　金：甘肃省自然基金项目(1208RJZA280);甘肃省科技成果转化项目(GNCX-2011-36)

摘　　要：试验选用抗寒山葡萄‘双红’和‘双优’2个品种的茎段为外植体,分别从外植体的选择、消毒时间、不同激素配比等对不定芽诱导、增殖培养和生根培养效应等方面进行了研究,并确定驯化移栽的最适宜基质.结果表明:山葡萄‘双红’和‘双优’2个品种的外植体茎段,先用75%的酒精消毒30s、再用0.1%的HgCl2溶液消毒8min获得的外植体消毒效果最佳,成活率分别为86.67%和90.00%;最适宜的增殖培养基为GS+NAA 0.1mg/L,增殖倍数可达到4.00;最适宜的生根培养基为GS+IAA 0.05mg/L,可使单株平均根条数达9条以上,平均根长度达5cm以上,最适宜的移栽基质为细沙∶珍珠岩∶蛭石=1∶1∶2,移栽试管苗成活率达70.00%以上.The stems of Vitis amurensis two varieties ‘Shuanghong’and ‘Shuangyou’which have cold resistance were chosen as raw material.The influence of adventitious buds induction,proliferation and roo-ting culture,and the determination of the most appropriate matrix in transplanting stage were explored through several aspects including the choice of explants,sterilization time and the ratio of the different hor-mone.The results showed that the sterilization effect was best when the stems were disinfected with 75%alcohol first and then sterilized for 8 min in 0.1% HgCl2 solution.Survival rates were 86.67% and 90.00%for ‘Shuanghong’and‘Shuangyou’.The optimizing medium for proliferation of test tube plantlets was GS＋NAA 0.1 mg/L,value multiples could be achieved 4.00.The best rooting medium was GS＋IAA 0.05 mg/L,the root number could be over 9 per plant.The average length of adventitious roots was above 5 cm. The best matrix for transplanting plantlets was sand∶perlite∶vermiculite=1∶1∶2,survival rate up to 70.00%.

关 键 词：山葡萄 外植体 快速繁育 移栽 

分 类 号：S663.1[农业科学—果树学]