HPLC同时测定雪松松针中没食子酸、原儿茶酸和儿茶素的含量  

Simultaneous Determination of Gallic Acid, Protocatechuic Acid and Catechin in Pine Needles of Cedrus Deodara by HPLC

作　　者：邓毅[1] 宁红霞[1] 沈薇[2] 石晓峰[1,2] 李师[1] 雷艳萍[1] 

机构地区：[1]甘肃中医学院药学院,兰州730030 [2]甘肃省医学科学研究院药物研究所,兰州730050

出　　处：《中国现代应用药学》2014年第9期1104-1107,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：甘肃省科技支撑计划项目(1204FKCA152);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金项目(zzy-2011-05);甘肃省卫生厅中医药科研项目(GZK-2012-29)

摘　　要：目的建立同时测定雪松松针中没食子酸、原儿茶酸和儿茶素含量的高效液相色谱法。方法采用TC-C18（250 mm×4.6 mm,5μm）色谱柱,流动相为甲醇（A）-0.2%磷酸水溶液（B）梯度洗脱（0~8 min,10%A,8~45 min,10%→30%A）,流速：1.0 mL·min-1,检测波长：280 nm,柱温：25℃。结果雪松松针中没食子酸、原儿茶酸和儿茶素依次在0.4~4.0,4~40和2~20μg·mL-1内呈良好线性关系,相关系数r分别为0.999 8,0.999 9,0.999 9,加样回收率（n=9）分别为104.7%,99.5%和105.8%。结论该方法适用于雪松松针中没食子酸、原儿茶酸和儿茶素的含量测定,方法简单、快速、高效。OBJECTIVE To develop an HPLC method for simultaneous determination of gallic acid, protocatechuic acid and catechin components in pine needles of Cedrus deodara. METHODS Agilent TC-C18 column （250 mm×4.6 mm,5μm） was adopted. The mobile phase was methanol （A）-0.2% phosphoric acid （B） with gradient elution （0-8 min, 10%A; 8-45 min, 10%→30%A） at the flow rate of 1.0 mL.min-1. The column temperature was 25 ℃ and the detection wavelength was 280 nm. RESULTS Calibration curves were found to be linear in the ranges of 0.4-4.0 μg.mL-1 for gallic acid, 4-40 μg.mL-1 for protocatechuic acid and 2-20 μg.mL-1 for catechin. The correlation coefficients were 0.999 8, 0.999 9, 0.999 9. The average recoveries（n=9） of gallic acid, protocatechuic acid and catechin were 104.7%, 99.5% and 105.8%. CONCLUSION The method is simple, rapid and efficient, which is suitable for the determination of gallic acid, protocatechuic acid and catechin components in pine needles of Cedrus deodara.

关 键 词：雪松松针 没食子酸 原儿茶酸 儿茶素 含量测定 高效液相色谱法 

分 类 号：R917.101[医药卫生—药物分析学]