限食与不饱和脂肪酸对大鼠实验性结肠炎的影响  

Effects of combined calories restriction and polyunsaturated fatty acids on colitis in rats

作　　者：钱艳[1] 张英[1] 刘慧[1] 王蕾[1] 李秀花[1] 邱服斌[1] 

机构地区：[1]山西医科大学公共卫生学院营养与食品卫生学教研室,太原030001

出　　处：《卫生研究》2014年第5期793-796,804,共5页Journal of Hygiene Research

摘　　要：目的探讨限食(CR)和n-6、n-3多不饱和脂肪酸(PUFA)对大鼠溃疡性结肠炎模型的影响,以制备溃疡性结肠炎。方法 40只SD雌性大鼠按体重随机分为5组,即对照组、模型组、限食组、5∶1 PUFA自由进食组、5∶1 PUFA限食组。限食组喂食量是自由进食组的60%。喂养14周后,除对照组外,其余组给予5%葡聚糖硫酸钠(DSS)水溶液,自由饮用7 d造模。结果与自由进食组相比,限食组体重增长缓慢,尤其是5∶1PUFA限食组大鼠体重最轻(P<0.05)。与模型组比,5∶1 PUFA限食组大鼠的血清MPO活性、疾病活动指数、结肠大体和组织病理学评分降低(P<0.05)。与限食组比较,5∶1 PUFA限食组大鼠血清IL-6和TNF-α含量均降低,而IL-10含量升高(P<0.05)。结论膳食中添加n-6与n-3比例为5∶1的PUFA和限食联合作用对DSS诱导的大鼠结肠炎有明显保护作用。Objective To explore the effect of n-6 and n-3 polyunsaturated fatty acids combined with calorie restriction（CR） in DSS induced ulcerative colitis rats. Methods Forty female rats were randomly divided into five groups, control group, model group, CR group, 5：1 PUFA ad libitum group, 5：1 PUFA CR group. CR groups provided with a limited daily food allotment of 60% of that eaten by the ad libitum animals for 14 weeks. Ulcerative colitis model in rats were given 5.0% dextran sulfate sodium in their drinking water for 7 days. Results 5 ： 1 PUFA CR group significantly decreased body weight, disease activity index, macroscopic and histological score compared to model group. In addition, administration of 5：1 PUFA CR effectively inhibited MPO activity. The levels of TNF-α and IL-6 in the serum with colitis were decreased by 5：1 PUFA CR （P 〈 0.05）. Conclusion These results suggest that combination of calories restriction and n-6/n-3 = 5：1 PUFA may be more beneficial in attenuating the progression of DSS induced ulcerative colitis.

关 键 词：多不饱和脂肪酸 限食 溃疡性结肠炎 TNF-α IL-6 IL-10 

分 类 号：R574.62[医药卫生—消化系统] R151.3[医药卫生—内科学]