蒜头果蛋白对人白血病K562细胞体外生长的抑制作用  

The inhibitory effect of malanin on the growth of human leukemia K562 cells in vitro

作　　者：袁燕[1] 张薇[1] 李彬[1] 王红斌[1] Colin J.Barrow 杨文荣[2] 

机构地区：[1]云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明650500 [2]澳大利亚迪肯大学生命与环境科学学院化学与生物技术中心,澳大利亚吉朗3216

出　　处：《食品工业科技》2014年第20期379-382,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金(81260500);云南省教育厅重点项目(2011Z047);教育国际化平台建设项目(218-02001001002129);云南省无机化学精品课程(2012HX08,2012HX12)

摘　　要：从蒜头果种子中提取蒜头果蛋白并测定其对人白血病K562细胞体外生长的抑制作用。结果表明,蒜头果蛋白具有明显抑制K562细胞体外增殖的作用,且存在时间和剂量依赖性,其半数抑制浓度IC50值为1.58×10-8mol·L-1。蒜头果蛋白与顺铂联用表现出了明显的协同抑制效应,两药联用效应会增加。此外,蒜头果蛋白的热稳定性较好,非常便于贮存。本文为蒜头果蛋白在肿瘤治疗方面的开发利用提供依据和思路,也为该蛋白作为新药的研发奠定基础。A novel protein named malanin was purified from the seeds of Malania oleifera,and its inhibitory effect was determined on the growth of human leukemia K562 cells in vitro. The result showed that malanin could significantly inhibit the viability of K562 cells in a time and dose-dependently manner,producing the half inhibitory concentration IC50 value of 1.58 xl0^4mol· L-1. Malanin combined with cisplatin showed a significant synergistic inhibitory effect,and the effect of two drugs combined treatment would increase. In addition, malanin had good thermal stability and very easy storage. It provided a basis and ideas for malanin on development and utilization in cancer therapy,and it was also to lay the foundation for the protein as the research and development of new drugs.

关 键 词：蒜头果蛋白 人白血病K562细胞 抗肿瘤活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]