N-琥珀酰低聚壳聚糖的抗氧化性能研究(英文)  

Antioxidant Activity of N-Succinyl Chitosan Oligosaccharides

作　　者：李铭[1] 熊小英[1] 谢晶[1] 薛斌[1] 银旭红[1] 孙涛[1] 

机构地区：[1]上海海洋大学食品学院上海水产品加工储藏工程研究中心,上海201306

出　　处：《天然产物研究与开发》2014年第10期1680-1684,共5页Natural Product Research and Development

基　　金：National"Twelfth Five-Year"Plan for Science&Technology Support(2011BAD24B02);Engineering Research Center of Shanghai Committee of Science and Technology(11DZ2280300)

摘　　要：低聚壳聚糖经化学改性得到三种取代度不同的N-琥珀酰低聚壳聚糖(NSCOSA、NSCOSB和NSCOSC,取代度(DS)分别是为0.25、0.57和0.70。对其结构进行红外表征,并且并考察了其对超氧阴离子自由基(O-·2)、羟基自由基(·OH)、DPPH的清除活性以及还原能力。结果表明:N-琥珀酰低聚壳聚糖衍生物的抗氧化活性与低聚壳聚糖相比有所降低。随着DS的升高,琥珀酰衍生物对·OH的清除能力下降;对O-·2的清除能力大小顺序为NSCB>NSCA>NSCC;对DPPH的清除能力以及还原能力大小顺序均为:NSCA>NSCC>NSCB。这可能是由于N-琥珀酰低聚壳聚糖的取代度、引进基团的性质以及对自由基的清除机理不同所致。Three N-suecinyl chitosan oligosaccharides （NSCOSs） with different substituting degrees （ NSCOSA：0.25, NSCOSB ：0.57 and NSCOSC ：0.70, respectively） were prepared by reacting of chitosan oligosaccharide （COS） and sue- cinie anhydride. The structure changes of the COS derivatives were characterized by Fourier transform infrared （FT-IR） spectra. Their antioxidant activities were evaluated by the scavenging of superoxide radical O2- , hydroxyl radical · OH, 1,1-diphenyl-2-plcrylhrasyl radical （DPPH） and determination of reducing power. The results indicated that all the NS- COSs showed obvious antioxidant activity, the orders of their scavenging effect on O2- was NSCOSB 〉 NSCOSA 〉 NS- COSC, the scavenging effect on · OH decreased with the increasing of substituting degrees （ DSs）, the orders of their scavenging effect on DPPH and reducing power both were： NSCOSA 〉 NSCOSC 〉 NSCOSB. The result may be related with the substituting degrees, the properties of the substituting group and the different scavenging mechanisms against free radicals.

关 键 词：低聚壳聚糖 N-琥珀酰低聚壳聚糖 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]