乌骨鸡黑色素氧化降解工艺研究  

Research on Oxidative Degradation Technology of Melanin from Black-Bone Silky Fowl

作　　者：黄宇玫[1,2] 田颖刚[1] 乔娟娟[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌330047 [2]南昌大学科学技术学院,南昌330029

出　　处：《天然产物研究与开发》2014年第10期1705-1710,共6页Natural Product Research and Development

基　　金：国家自然科学基金项目(20962015);江西省自然科学基金项目(2009GZN0087);江西省教育厅科技项目(GJJ10308)

摘　　要：采用碳酸钾-过氧化氢法、高锰酸钾法、氢氧化钠-过氧化氢法及氨水-过氧化氢法对乌骨鸡黑色素进行氧化降解,并获得可溶性的降解产物,运用GC-MS分析降解萃取产物中物质种类,并且分析了降解产物得率和分子量分布,发现降解方法不同,降解产物各指标也存在相应差异。其中高锰酸钾法得率最高为82.5%,碳酸钾-过氧化氢次之为61.5%,其余均在50%以上;降解产物分子量以氨水-过氧化氢法最高,高达12265.5 Da,其降解程度最小,其萃取物中的物质种类及含量较少,而强酸性环境下高锰酸钾氧化降解乌骨鸡黑色素程度最大,降解产物中存在许多被彻底氧化的有机酸类,进一步表明强酸、强碱介质中黑色素更容易被氧化断裂。The Black-bone silky fowl melanin （BSFM） was degraded with K2CO3-H2O2 ,KMnO, ,NaOH-H2O2 and NH3 · H2O-H2O2 oxidative degradation methods to afford water-soluble products. The identities and molecular weight distri- butions of the degradation products were analyzed and the degradation yields of different methods were calculated. Re- suits showed that products from different degradation methods were different. The degradation yield of KMnO4-degrada- tion method was up to 82. 5%, followed by K2CO3-H2O2 method with 61.5%, the degradation yields of the rest two methods were also above 50%. As to the molecular weight of degradation products, NI-I3 ~ H202 degradation products was the biggest and up to 12265.5 Da,the degradation degree was least,the types and quantities of NH3 · H2O-H2O2 degradation products was also less than the other three methods. On the other hand,with the strong acidic environment, KMnO4 oxidation degradation degree of BSFM was largest. It further proved that in the strong acidic and/or basic condi- tions melanin was more easily to be oxidized and hence degraded.

关 键 词：乌骨鸡黑色素 氧化降解 分子量 GC-MS 

分 类 号：R914[医药卫生—药物化学]