产褐藻胶裂解酶菌株Cobetia sp. WG-007的筛选及发酵优化  

Screening of Alginate Lyase Producing Strain Cobetia sp. WG-007 and Optimization of Fermentation

作　　者：刘旭梅[1] 李恒[1] 蒋敏[1] 龚劲松[1] 许正宏[1] 史劲松[1] 

机构地区：[1]江南大学药学院,江苏无锡214122

出　　处：《食品与生物技术学报》2014年第9期903-909,共7页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(21206055);国家"十二五"科技支撑计划项目(2012BAD33B06);国家863计划项目(2012AA022204C)

摘　　要：以褐藻酸钠为唯一碳源,从腐烂的海带中筛选出一株高效降解褐藻胶的菌株,经形态、生理生化和16S rDNA测序鉴定,将其命名为Cobetia sp.WG-007。采用单因素和正交试验优化,确定该菌株摇瓶发酵最适产酶条件为褐藻酸钠6.0 g/L,蛋白胨5.0 g/L,NaCl 20 g/L、K2HPO40.25 g/L,菌株在25℃条件下发酵培养30 h达到最高酶活,为160.7 U/mL,相比优化前酶活力提高了3.52倍。A high efficient alginate-degrading strain was isolated from rotten seaweed using sodium alginate as the sole carbon source.Based on the physiological characteristics as well as 16S rDNA sequencing,the strain was identified as Cobetia sp.WG-007.The optimized fermentation medium was composed of sodium alginate 6.0 g/L,peptone 5.0 g/L,NaCl 20 g/L,K2HPO4 0.25 g/L.The strain was cultivated at 25 ℃ for 30 h under the optimized conditions and the alginate lyase activity was increased to 160.7 U/mL,which was 3.52-fold compared with the control.

关 键 词：褐藻胶裂解酶 Cobetia sp.WG-007菌株 筛选 发酵 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]