不同产地桃金娘果中5种酚类成分的测定及其抗氧化作用研究  

Determination of five phenolic compounds from fruits of Rhodomyrtus tomentosa in different regions and its antioxidant effect

作　　者：肖婷[1,2] 崔炯谟[1] 郭正红[2,3] 赵余庆[2,3] 

机构地区：[1]延边大学药学院,吉林延吉133000 [2]沈阳药科大学中药学院,辽宁沈阳110016 [3]沈阳药科大学教育部基于靶点药物设计与研究重点实验室,辽宁沈阳110016

出　　处：《中草药》2014年第18期2703-2706,共4页Chinese Traditional and Herbal Drugs

基　　金：中药标准提取物工业化色谱制备技术子平台(2010ZX09401-304-105-002)

摘　　要：目的采用HPLC测定6种不同产地的桃金娘果中5种酚类成分,并通过考察5种酚类成分和不同产地桃金娘果提取物清除自由基能力。方法 Inertsil ODS-SP色谱柱(150 mm×4.6 mm,5μm),流动相乙腈-1%磷酸水,梯度洗脱,检测波长为280 nm,体积流量1 mL/min,进样量10μL;采用DPPH法测定没食子酸、原儿茶酸、儿茶素、鞣花酸、白藜芦醇及6个不同产地桃金娘果提取物清除自由基的能力。结果 5种酚类成分线性关系良好,精密度、稳定性、重复性、加样回收率均符合要求;5种酚类成分和不同产地桃金娘果提取物清除自由基能力较好,广东新丰县、广东翁源县、广西南宁、云南、广西桂林、贵州产地桃金娘果提取物的IC50值分别为58.82、61.57、17.73、7.91、14.77、8.11μg/mL,没食子酸、原儿茶酸、儿茶素、鞣花酸、白藜芦醇的IC50值分别为0.02、0.04、0.03、0.05、5.7μg/mL,阳性对照VC的IC50值为6.31μg/mL。结论初步明确了桃金娘果清除自由基、抗氧化活性的物质基础,为桃金娘资源的综合利用和开发研究提供科学依据。Objective Five phenolic compounds in fruits of Rhodomyrtus tomentosa from six different regiones were measured by HPLC, and the ability of scavenging free radicals of five kinds ofphenolics and myrtle fi＇uits extracts in different regions was investigated. Methods The determination was performed on Inertsil ODS-SP column （150 mm ~ 4.6 mm, 5 ~tm）, the mobile phase consisted of acetonitrile and 0.1% phosphate aqueous solution with gradient elution program, flow rate was 1 mL/min with 10 pL of sample quantity at 280 nm, and the antiradical efficiency of five phenolic compounds and extrcts were evaluated by DPPH radical scavenging assay. Results Five kinds of phenolic components showed a good linear relationship, and the precision, stability, repeatability, and sample recovery rate all met the requirements. Five phenolic compounds and extracts had good antiradical efficiency. The IC50 values of the myrtle fruits extracts from Xinfeng, Wengyuan counties in Guangdong province, Narming, Guilin in Guangxi province, Yunnan province, and Guizhou province were 58.82, 61.57, 17.73, 7.91, 14.77, and 8.11 μg/mL, and the ICso values of gallic acid, protocatechuic acid, catechin, ellagic acid, and resveratrol were 0.02, 0.04, 0.03, 0.05, and 5.70 μg/mL, respectively. The IC50 of positive control VC was 6.31 μg/mL. Conclusion Material basis of antiradical efficiency of fruits is preliminary identified and it provides a scientific basis for the comprehensive utilization of the resources ofR. tomentosa.

关 键 词：桃金娘 酚类成分 DPPH法 清除自由基 HPLC 

分 类 号：R286.022[医药卫生—中药学]