昆虫JHE基因结构及与甲基化相关的CpG O/E值分析  

Insect JHE gene structure and analysis of CpG O /E value related with methylation

作　　者：张梅[1] 周晓穗[1] 陈佳林[1] 梁士可 李广宏[1] 王方海[1] 

机构地区：[1]中山大学生命科学学院昆虫学研究所/有害生物控制与资源利用国家重点实验室,广州510275

出　　处：《环境昆虫学报》2014年第5期705-710,共6页Journal of Environmental Entomology

基　　金：国家自然科学基金项目(31171844);广东省自然科学基金项目(S2011010001353)

摘　　要：本研究选择西方蜜蜂Apis mellifera、黑腹果蝇Drosophila melanogaster、致倦库蚊Culex quinquefasciatus和家蚕Bombyx mori 4种昆虫为例,以降解保幼激素的特异性酯酶(juvenile hormone esterase,JHE)为研究对象,首先运用NCBI的Spidey在线软件将各昆虫的JHE基因与其相应的基因组序列作比对,分别确定各昆虫JHE基因的上游2000 bp的具体序列和外显子及内含子区域。发现西方蜜蜂JHE基因含有7个外显子和6个内含子,而其余三种昆虫的JHE基因均含有6个外显子和5个内含子。接着,分别统计基因各区域的CpG,G,C位点的占有量,并计算出CpG O/E值(CpG位点的实际值与期望值之间的比值),发现各区域CpG O/E平均值的大小依次为:基因上游2000 bp区>内含子区>外显子区,基因上游2000 bp区的CpG O/E平均值大于1.0,而第1到第4外显子的CpG O/E平均值均只有0.8左右。表明在昆虫JHE基因中,若有CpG甲基化位点发生,应主要发生在第1到第4外显子区域。This research selected 4 kinds of insects, Apis mellifera, Drosophila melanogaster, Culex quinquefasciatus and Bombyx mori to analyze the structures and CpG O/E value of juvenile hormone esterase （JHE） genes. First, the JHE genes were compared with their corresponding genomic sequences by using Spidey software online of NCBI to identify the 2000 bp upstream sequence, exon and intron regions of these genes. The results showed that the JHE gene of Apis mellifera contained 7 exons and 6 introns, while the JHE genes of remaining three insects contained 6 exons and 5 introns. Then, analyzed the JHE gene regional share of CpG, G, C, CpG, and calculated CpG O/E values （the ratio between actual value and expected value of CpG locus）. The average values of CpG O/E in every area of JHE genes were found in the following order： gene upstream 2000 bp 〉 intron 〉 exon, and the average value of CpG O/E in gene upstream area 2000 bp was higher than 1, while the values in 1 s, _ 4th exon were 0. 8 or so. This implied that if there were the CpG methylation sites in insect JHE genes, they should mainly happen in the 1 st _4th exon regions.

关 键 词：保幼激素酯酶 DNA甲基化 基因结构 CPG O/E值 外显子 

分 类 号：Q965[生物学—昆虫学] S433.89[农业科学—农业昆虫与害虫防治]