多功能半纤维素酶的构建及其酶解应用分析  

Constructing Trifunctional Hemicellulase and Degradation of Arabinoxylan

作　　者：彭静静[1] 

机构地区：[1]泰山学院生物与酿酒工程学院,山东泰安271021

出　　处：《湖北农业科学》2014年第16期3933-3935,3951,共4页Hubei Agricultural Sciences

基　　金：泰安市科技发展计划项目(20132094);泰山学院博士科研启动金项目(Y-01-2013001)

摘　　要：以前期构建的来源于嗜热厌氧乙醇菌(Thermoanaerobacter ethanolicus JW200)的双活性阿拉伯/木糖苷酶(XarB)和来源于疏棉状嗜热丝孢菌(Thermomyces lanuginosus DSM 5826)木聚糖酶A(XynA)融合酶为基础,在融合酶的两个催化结构域间插入多肽Linker,并通过优化Linker组成和长度避免催化结构域互相之间的干扰,以增强融合酶的催化效率。通过酶解燕麦木聚糖和麦麸试验发现,带有多肽Linker的融合酶催化效率得到了提高。The trifunctional enzyme （XarB-S-XynA） associatied with xylosidasearabinosidase （XarB） of Thermoanaerobacter ethanolicus and xylanase （XynA） of Therrnomyces lanuginosus was produced in E. coli to study the effects of the physical association of the fusion partners on the enzymatic efficiency. Recombinants XarB, XynA and XarB-S-XynA were purified to homogeneity and characterized. The fusion enzyme was inserted between the two catalytic domains of the polypeptide linker. Mutual interferences between the catalytic domain were avoided to enhance the catalytic efficiency of the fusion enzyme by optimizing the composition and length linker. The trifunctional hemicellulase was tested for degradating oat spelt xylan and wheat bran. Catalytic efficiency of trifunctional enzyme was improved by poly- peptide Linker.

关 键 词：半纤维素 阿拉伯/木糖苷酶 木聚糖酶 热激表达载体pHsh 酶解 

分 类 号：Q78[生物学—分子生物学]