生姜提取物对邻苯三酚自氧化生成超氧自由基的清除  

Antioxidant Capacity of Ginger Active Ingredients by Pyrogallol Auto-oxidation Action

作　　者：程德竹 杜爱玲[1] 李成帅[1] 杜爱琴[1] 

机构地区：[1]山东大学化学与化工学院,济南250061

出　　处：《中国调味品》2014年第11期35-39,共5页China Condiment

摘　　要：通过检测生姜提取物对邻苯三酚自氧化反应生成的超氧自由基的清除,表征其抗氧化能力。首先确定邻苯三酚自氧化反应条件,然后加入多种生姜提取物,检测其对超氧自由基的清除效果。检测方法为测反应产生的半醌在320 nm 处的紫外吸光度。结果表明邻苯三酚自氧化反应条件为：反应温度24℃,体系 pH 8.40-8.52,反应时间17-18 min。各类提取物中,以姜酚为主的粗分物对超氧自由基的清除率最高,清除率与提取物浓度呈线性关系。相同浓度下,超临界 CO2萃取分离物对超氧自由基的清除率明显高于相应的溶剂萃取分离物。The antioxidant capacity of ginger active ingredients is characterized by the ability of scavenging superoxide radical,which is produced by pyrogallol auto-oxidation action.The experiment factors of pyrogallol auto-oxidation action including temperature,reaction time,and pH are firstly researched.Then the ginger extracts are added into the mixture to scavenge the superoxide radical. The detection method is measuring the UV absorbance value of semiquinone at 320 nm.The results show that the appropriate conditions are：24 ℃,17-18 min,and pH 8.40-8.52.The samples with gingerol as the main ingredient have the highest scavenging ratio of superoxide radical,and the scavenging ratio has a linear relationship with the extraction concentration.The scavenging ratio of supercritical CO2 extraction is obviously higher than solvent extraction under the same mass concentration.

关 键 词：生姜分离物 超氧自由基 邻苯三酚 抗氧化性 

分 类 号：TS264.2[轻工技术与工程—发酵工程]