冬虫夏草菌丝体发酵液中多糖的抗氧化活性研究  

Antioxidant Activity of Polysaccharide in Fermentation Broth of Cordyceps Sinensis

作　　者：赵萍[1] 曹心张 张新国[1] 刘英娟[1] 刘冰[1] 张春生[1] 寇飞[1] 袁亚兰[1] 

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《中医药学报》2014年第5期27-29,共3页Acta Chinese Medicine and Pharmacology

基　　金：甘肃省重点学科建设项目资助

摘　　要：用苯酚-硫酸法测定多糖含量,DPPH法和总还原力法测定抗氧化活性,对冬虫夏草菌丝体发酵液胞外多糖(CSFB)的含量与抗氧化活性的关系、37℃两倍浓缩发酵液CSFB37和60℃两倍浓缩发酵液CSFB60的活性进行了研究。结果表明,CSFB多糖含量为1.28mg/mL,CSFB37和CSFB60的多糖含量分别为2.85mg/mL、2.50mg/mL;CSFB、CSFB37和CSFB60多糖具有清除DPPH自由基能力和还原力能力,CSFB、CSFB37和CSFB60的DPPH的IC50值分别为4.49μg/mL、5.54μg/mL和5.30μg/mL;多糖抗氧化活性大小为:CSFB>CSFB60>CSFB37。Use Phenol-sulfuric acid to measure the content of polysaccharide and the method of DPPH and general re-ducing force to test antioxidant activity of Fermentation Broth of Cordyceps sinensis.This paper had carried out a research on the relationship of the content of exopolysaccharides and antioxidant activities of them and compared the activities of the twice fold concentrated Fermentation Broth CSFB37 and CSFB60 under the condition of 37℃ and 60℃, respectively. The results showed that contents of exopolysaccharides of CSFB, CSFB37 and CSFB60 were 1.28mg/mL,2.85 mg/mL and 2.50 mg/mL;CSFB, CSFB37 and CSFB60 were of the abilities of clearing the free radical of DPPH and reducing pow-er, CSFB,CSFB37 and CSFB60 IC50 were 4.49μg/mL、5.54μg/mL and 5.30μg/mL;and the antioxidant activity of poly-saccharide was CSFB〉CSFB60 〉CSFB37 .

关 键 词：冬虫夏草 胞外多糖 DPPH 总还原力 抗氧化活性 

分 类 号：R285[医药卫生—中药学]