二次发酵提取茶皂素的方法研究  

Method of Abstracting Tea-saponin with Secondany Fermemtation

作　　者：王文杰[1] 陈长庚[1] 张必桦[1] 黄建琴 吴新荣[1] 徐奕鼎[1] 方吴云[1] 王烨军[1] 雷攀登[1] 吴琼[1] 

机构地区：[1]安徽省农业科学院茶叶研究所,安徽祁门245600

出　　处：《中国农学通报》2014年第30期296-301,共6页Chinese Agricultural Science Bulletin

基　　金：国家星火计划子课题"标准茶园高效栽培关键技术集成与应用"(2012GA710001);安徽省农科院创新团队建设项目"茶树种质资源创新与利用"(11C0809);安徽省农科院农业科技创新项目"茶皂素对茶尺蠖毒性作用机理研究"(13B0838)

摘　　要：为提取饼粕中的茶皂素,根据饼粕中含有较多糖类物质的特点,对饼粕进行酒精发酵研究。结果表明:采用二次发酵法,可以成功地促进饼粕的酒精发酵,每百克饼粕因酒精发酵而被消耗12.58 g糖类物质;茶皂素的提取率达80%左右,且粗提茶皂素纯度高,可以达到62.37%;玉米粉与饼粕的配比为1:1.5较为合适;浓缩能耗低,初提液茶皂素浓度可达78.26 mg/m L。Alcohol fermentation was carried out to extract tea-saponin based on the feature that there are a lot of saccharides substances in tea cake. The result showed that secondary fermentation could successfully promote alcohol fermentation. 12.58 g carbohydrate per 100 g cake was consumed during alcohol fermentation. The extraction percentage could reach 80%, and the purity of crude extraction could achieve 62.37%. It was appropriate when the proportioning of corn meal and cake was 1：1.5. The energy consumption of concentration was low. The concentration of tea-saponin in original extracts was 78.26 mg/mL.

关 键 词：茶皂素 二次发酵 提取率 玉米粉配比 

分 类 号：S-3[农业科学]