大蒜素提高人胰腺癌BXPC3细胞放射敏感性的机制研究  

Effect of allicin on the radiosensitivity of human pancreatic carcinoma BXPC3 cells

作　　者：马红兵[1] 狄政莉[2] 何娜[3] 问娇 柯悦 

机构地区：[1]西安交通大学第二附属医院肿瘤病院肿瘤放疗科,710004 [2]西安市中心医院神经内科 [3]西安交通大学第二附属医院眼科,710004

出　　处：《中华放射医学与防护杂志》2014年第10期743-747,共5页Chinese Journal of Radiological Medicine and Protection

基　　金：陕西省科技攻关项目（2010k01-141）

摘　　要：目的 观察大蒜素对人胰腺癌BXPC3细胞的生长及放射敏感性的影响.方法 大蒜素联合X射线处理胰腺癌BXPC3细胞,采用MTT法检测细胞的生长和增殖;细胞流式技术测定细胞周期改变以及细胞凋亡;细胞克隆形成法观察大蒜素对辐射的增敏作用.采用RT-PCR、Western blot分析细胞中Bax、Bcl-2表达变化.结果 12、24和48 h大蒜素处理导致细胞生长抑制的半数抑制浓度（IC50）分别为76.24、58.34和43.58μmol/L.与单纯照射组相比,照射联合应用大蒜素明显抑制胰腺癌细胞的生长（=2.74,P＜0.05）,影响细胞周期的变化,显著增加G2/M期细 胞阻滞（t=11.41,P＜0.05）;细胞凋亡率明显提高（t=12.36,P＜0.05）,伴有Bax表达的增高（t=4.83,P＜0.05）和Bcl-2的表达下调（=3.69,P＜0.05）. 结论 大蒜素可以通过诱导细胞周期阻滞及细胞凋亡而抑制胰腺癌BXPC3细胞的生长,具有放射增敏作用,凋亡相关因子Bax、Bcl-2参与了这一过程.Objective To study the effect of allicin on the growth and radiosensitivity of human pancreatic carcinoma BXPC3 cells.Methods BXPC3 cells were exposed to X-rays in the presence or absence of allicin.Cell proliferation was measured by MTT assay.Cell cycle distribution and apoptosis were detected by flow cytometry assay.Cell radiosensitivity and the influence of allicin on it was evaluated by colony formation assay.The expressions of Bax and Bcl-2 proteins were assayed by RT-PCR and Western blot.Results IC50 values of allicin on cell growth were 76.24,58.34 and 43.58 μmol/L under 12,24 and 48 h treatment,respectively.Treatment of cells with allicin obviously inhibited cell growth after irradiation and hence increased radiosensitivity （t =2.74,P 〈 0.05）.This treament also enhanced radiation-induced cell cycle arrest at G2/M phase （t =11.41,P 〈0.05）,apoptosis induction （t =12.36,P 〈 0.05）,and Bax expression （t =4.83,P 〈 0.05）,but it decreased Bcl-2 expression （t =3.69,P 〈 0.05）.Conclusions Allicin could inhibit cell growth,induce cell cycle arrest and apoptosis via Bax/Bcl-2 pathway and hence increases radiosensitivity of BXPC3 cells.

关 键 词：大蒜素 BAX BCL-2 放射敏感性 胰腺癌 细胞凋亡 

分 类 号：R735.9[医药卫生—肿瘤] R730.55[医药卫生—临床医学]