白酒丢糟的多酶复配降解制备可发酵性糖  

Preparing of fermentable sugars from pretreatment Chinese liquor distillers' grains by using multi-enzyme hydrolysis

作　　者：刘跃红[1] 吴正云[1] 杨健[1] 吴艳萍[1] 袁玉菊[1] 张文学[1,2] 

机构地区：[1]四川大学食品工程系,成都610065 [2]四川大学锦江学院白酒学院,眉山620860

出　　处：《生物加工过程》2014年第6期18-22,共5页Chinese Journal of Bioprocess Engineering

基　　金：科技部国际合作项目(2009DFA60890);四川省科技支撑计划(2013GZX0161)

摘　　要：为有效利用纤维质原料制备可发酵性糖生产燃料酒精,通过NaOH 过氧乙酸预处理白酒丢糟,经质量分数2%NaOH 和体积分数6%过氧乙酸处理后,白酒丢糟中木质素去除率达66. 13% ～ 77. 02%,总纤维素回收率78. 04%～90. 73%.对处理后的白酒丢糟进行多酶复配糖化降解,通过均匀设计实验确定白酒丢糟酶降解的数学模型,得出总糖降解率与各酶添加量之间的回归关系,在最优条件下白酒丢糟降解率为（0. 432 8±0. 013 5） g/ g.We used NaOH-peracetic acid pretreatment of distillers′ waste grains to convert holocellulose tofermentable sugars for bioethanol production. The results show that when the distillers′ grains is pretreatedwith a mixture of 2% （m / m） NaOH solution and 6% （V / V） peracetic acid solution,66. 13% to 77. 02%lignin was removed and 78. 04%-90. 73% cellulose was recovered.The experiment of multienzymehydrolysis with multiple factors and multiple levels was designed by the date processing system accordingto U8（85）. The mathematic model for total sugars degradation rate was acquired and it showed that underthe optimal condition,the total sugars degradation rate was （0. 432 8±0. 013 5） g/ g.

关 键 词：NaOH过氧乙酸 白酒丢糟 多酶复配 均匀设计 

分 类 号：TQ261[化学工程—有机化工]