参芎化瘀胶囊通过VEGF/Notch1信号通路改善大鼠缺血性脑卒中损伤  

Shenxiong Huayu capsule attenuates cerebral ischemia injury by regulating VEGF/Notch1 signal pathway in rats

作　　者：赵雅宁[1] 刘文倩[1] 牛静[1] 李建民[2] 李淑杏[1] 陈长香[1] 

机构地区：[1]河北联合大学护理与康复学院,河北唐山063000 [2]河北联合大学附属医院神经外科,河北唐山063000

出　　处：《西安交通大学学报（医学版）》2014年第6期843-847,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：河北省科技厅课题基金(No.H2012401007)~~

摘　　要：目的探讨参芎化瘀胶囊对全脑缺血再灌注损伤大鼠的治疗作用及对海马区VEGF/Notch1通路的影响。方法 SD大鼠分为假手术组、模型组及参芎化瘀胶囊高、低剂量组。采用改良的Pulsineli 4血管阻断(4-VO)法制作全脑缺血模型,分别在缺血24、48、72h应用光镜观察海马区神经细胞形态的变化;应用免疫组化法检测海马区VEGF、Notch1表达。结果与假手术组比较,模型组海马区各时间点的存活神经细胞率降低,差异有统计学意义(P<0.05);VEGF、Notch1表达升高,差异有统计学意义(P<0.05)。与模型组比较,参芎化瘀胶囊组各时间点的存活神经细胞率增加,差异有统计学意义(P<0.05);VEGF、Notch1表达进一步升高,差异有统计学意义(P<0.05)。上述变化在高剂量参芎化瘀胶囊组更为明显。模型组VEGF与Notch1表达呈正相关(r=0.846,P<0.01);参芎化瘀胶囊组VEGF与Notch1表达呈正相关(r=0.814,P<0.01)。结论参芎化瘀胶囊对脑缺血损伤有很好的治疗作用,其机制可能与调控VEGF/Notch1信号途径有关。Objective To investigate the effects of Shenxiong Huayu capsules in treating cerebral ischemia- reperfusion and on vascular endothelial growth factor （VEGF）/Notchl signal pathway. Methods Male SD rats were divided randomly into four groups： sham operation group, model group, and Shenxiong Huayu capsule low- dose and high-dose treatment groups. Global cerebral ischemia model was created by improved four-vessel occlusion as described according to Pulsinelli＇s method. Morphological changes in the hippocampal region were observed by HE staining at 24 h, 48 h and 72 h after ischemia; VEGF and Notchl expressions were measured by immunohistochemistry. Results Compared with those in sham group, the survival rate of nerve cells decreased significantly at each time point （P〈0. 05） and VEGF and Notch1 expressions enhanced obviously in model group （P〈0. 05）. Compared with model group, the survival rate of nerve cells increased obviously at each time point and VEGF and Notchl expressions further increased in the two Shenxiong Huayu capsule groups （P〈0.05）. The above- mentioned changes were more significant in Shenxiong Huayu capsule high-dose group. VEGF and Notchl expressions had a positive correlation with each other in model group （r = 0. 846, P〈0.01） and Shenxiong Huayu capsule groups （r=0. 814, P〈0. 01）. Conclusion Shenxiong Huayu capsules have a good therapeutic effect on cerebral ischemia injury and the molecular mechanism is related to regulating VEGF/Notchl signal pathway.

关 键 词：参芎化瘀胶囊 脑缺血再灌注 血管内皮生长因子 NOTCH1 

分 类 号：R743[医药卫生—神经病学与精神病学]