块茎与块根多酚氧化酶部分性质的比较  

Comparision of Some Natures of Polyphenol Oxidase in Stem Tuber and Root Tuber

作　　者：雷晗枭 陈国伟[1] 李晓辉[1] 夏珊珊[1] 吴美仙[1] 

机构地区：[1]浙江海洋学院萧山科技学院,浙江杭州311215

出　　处：《安徽农业科学》2014年第33期11617-11618,共2页Journal of Anhui Agricultural Sciences

基　　金：2013年度浙江海洋学院大学生科技创新项目(自然科学类)(浙海院团〔2013〕38号);浙江省教育厅科研项目(Y201225035)

摘　　要：[目的]为高职学生实验《多酚氧化酶的制备和性质研究》筛选合适的实验材料。[方法]选取马铃薯和紫薯比较研究块茎与块根中多酚氧化酶(PPO)的活性、最适底物、最适温度等特性。[结果]两者PPO最适底物均为邻苯二酚;马铃薯PPO的最适反应温度为35℃,紫薯PPO的最适反应温度为10℃。[结论]经综合比较,该实验以马铃薯作为实验材料更为合适。[Objective] The objective of this study was to choose ideal experiment material for the students＇ experiments-The Making and Natures Study of Polyphenol Oxidase（PPO） in higher vocational education.[Method] The potato and purple potato were selected to study some natures of PPO,including its activity,optimum substrate and optimum reaction temperature.[Result] Both of their optimum substrates were Catechol.The optimum reaction temperature of potato was 35 ℃,while that of purple potato was 10 ℃.[Conclusion] After comprehensive comparison,potato was more ideal as experiment material than purple potato in this experiment.

关 键 词：马铃薯 紫薯 多酚氧化酶 活性 

分 类 号：S532[农业科学—作物学]