基于活的但非可培养细菌降解毒死蜱的研究  

Research on Viable but Non-culturable Bacteria in Chlorpyrifos Degradation

作　　者：吴咚咚[1] 王强[2] 蔡慧慧[1] 施洁莹[1] 付惠玲[1] 王新全[2] 周育[2] 丁林贤[1] 

机构地区：[1]浙江师范大学地理与环境学院,浙江金华321004 [2]浙江省农业科学院农产品质量标准研究所,浙江杭州310000

出　　处：《安徽农业科学》2014年第33期11711-11714,共4页Journal of Anhui Agricultural Sciences

基　　金：国家高技术研究发展计划(863)项目(2011AA100806);国家自然科学基金项目(31340071);浙江省自然科学基金项目(LY13C010002)

摘　　要：[目的]探索降解毒死蜱的新的微生物资源。[方法]应用最大或然数(MPN)法,通过添加适量复苏促进因子(Rpf)从多种土壤及污水生物处理系统中分离活的非可培养(VBNC)资源菌种,根据文献报道的已知毒死蜱降解菌株的系统关系综合分析,初步筛选出与已知降解毒死蜱菌种近缘的VBNC菌株并进行降解试验。[结果]共筛选出6株具有毒死蜱降解能力的菌株,其中菌株CHZYR63对毒死蜱的生物降解能力最强,经Box-behnken响应面法优化后降解率可达70.15%。[结论]可培养化VBNC菌种资源在残留农药降解方面有较大的利用价值,为揭示受染环境中VBNC微生物的形成及其活性化、环境污染物质的共代谢等微生物群体的相互作用机制提供了参考。[Objective] The aim was to explore new microbial resources to degrade Chlorpyrifos.[Method] Using MPN system,some strains of the VBNC（viable but non-culturable）bacteria which could degrade Chlorpyrifos were isolated from soil and sewage biological treatment system through adding with some Rpf （resuscitation promoting factor）.[Result] Total six strains of the VBNC were obtained,among them the biodegradability of strain CHZYR63 could reach 70.15％ through the analysis of Box-behnken response surface method.[Conclusion] The culturable VBNC bacteria not only have great value in degrading pesticide residues,but also provide important ideas and methods on revealing the formation mechanism and recovery mechanism of VBNC bacteria in polluted environment and total metabolism of environmental pollutants and interaction mechanism of the microbial population.

关 键 词：Rpf VBNC 毒死蜱 生物降解 响应面法 

分 类 号：S182[农业科学—农业基础科学] X172[环境科学与工程—环境科学]