HPLC测定六神曲中青蒿素、芦丁和槲皮素含量  

Determination of artemisinin, rutin and quercetin in Massa Medicata Fermentata by HPLC

作　　者：谢彦博[1] 徐云[2] 孙盛[2] 相宏宇[2] 刘洪章[1] 谢秋宏[2] 

机构地区：[1]吉林农业大学生命科学学院,吉林长春130033 [2]吉林大学生命科学学院,吉林长春130012

出　　处：《中国酿造》2014年第10期140-144,共5页China Brewing

基　　金：吉林省科技厅医药产业化推进项目(20130727036YY);吉林省科技厅重大科技攻关项目(20140203001YY,20140203024NY);重点攻关项目(20130206009SF)

摘　　要：本研究建立了高效液相色谱法测定六神曲中青蒿素、芦丁和槲皮素成分含量的方法。采用AcchromXAquaC18色谱柱(4.6mm×250 mm,5μm)。测定青蒿素含量时,用柱前衍生方法,以乙腈-水(60∶40)为流动相;流速0.8 mL/min,紫外检测波长为260 nm,柱温35℃。测定芦丁和槲皮素含量时,以甲醇-0.4%磷酸(50∶50)为流动相;紫外检测波长分别为360 nm(芦丁)和280 nm(槲皮素);流速0.8 mL/min;柱温30℃。结果青蒿素、芦丁和槲皮素可以达到较好的分离。在选定质量浓度范围内线性关系良好(青蒿素R2=0.9991,芦丁R2=0.9994,槲皮素R2=0.9995),平均回收率分别为98.6%,101.4%和97.2%,相对标准偏差(RSD)分别为1.65%,0.91%和1.46%(n=5)。该方法简便准确,重复性和稳定性较好,为六神曲的质量评价和控制提供了科学依据。HPLC method was described for determining the contents of artemisinin, rutin and quercetin in Massa Medicata Fermentata（MMF）. Chromatography separation was performed on HPLC system with Acchrom XAqua C18（4.6 mm×250 mm, 5 μm） column. Artemisinin was determined with the method of pre-column reaction and isocratic elution with mobile phase acetonitrile/water（60∶40）, the flow rate was 0.8 ml/min, and UV detection wavelength and temperature was 260 nm and 35 ℃, respectively. Rutin and quercetin were determined under the condition of mobile phase methanol/0.4% phosphoric acid（50∶50）, flow rate 0.8 ml/min, temperature 30 ℃, detection wavelength 360 nm for rutin and 280 nm for quercetin,respectively. The results indicated that the separation of artemisinin, rutin and quercetin was good. A good linearity was observed in the selected concentration range（artemisinin R2=0.999 1, rutin R2=0.999 4 and quercetin R2=0.999 5）. The average recoveries of the three compounds were 98.6%（RSD 1.65%）, 101.4%（RSD 0.91%） and 97.2%（RSD 1.46%）（n=5）, respectively. The method was simple with good accuracy and reproductivity and it can be applied for quality evaluation and control for Massa Medicata Fermentata.

关 键 词：六神曲 青蒿素 芦丁 槲皮素 高效液相色谱 

分 类 号：R282.71[医药卫生—中药学]