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作 者:梁晓维[1,2] 李发弟[1] 张军民[2,3] 马悦培[2,4] 赵青余[2,3] 高红亮[4]
机构地区:[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]中国农业科学院北京畜牧兽医研究所,农业部华北动物遗传资源与营养科学观测实验站,北京100193 [3]中国农业科学院-世界农用林业中心农用林业与可持续畜牧业联合实验室,北京100193 [4]华东师范大学,上海200062
出 处:《中国畜牧兽医》2014年第11期133-138,共6页China Animal Husbandry & Veterinary Medicine
基 金:中央级公益性科研院所基本科研业务费专项基金项目(2009yg-3);北京市大兴区与中国农业科学院科技合作项目
摘 要:试验将蒙脱石和凹凸棒石分别添加到含有黄曲霉毒素B1(AFB1)、玉米赤霉烯酮和T-2毒素的人工胃液或人工肠液中,探讨2种矿物对霉菌毒素的吸附作用。结果表明,蒙脱石和凹凸棒石对AFB1、玉米赤霉烯酮、T-2毒素的吸附均在1h达到平衡;钠基蒙脱石对AFB1和T-2毒素的吸附率高于钙基蒙脱石和凹凸棒石,吸附率分别为96.8%、38%;蒙脱石和凹凸棒石对玉米赤霉烯酮的吸附率均小于10%。pH对蒙脱石和凹凸棒石吸附霉菌毒素影响显著(P<0.05),pH 5时钙基蒙脱石对AFB1的吸附率显著高于pH 3、pH 7时(P<0.05),为90.1%;蒙脱石和凹凸棒石对玉米赤霉烯酮的吸附效果在pH 3时较佳,对T-2毒素的吸附效果在pH 7时显著高于pH 3、pH 5时(P<0.05)。蒙脱石和凹凸棒石对吸附的霉菌毒素均存在解吸现象,其中AFB1和玉米赤霉烯酮解吸明显。蒙脱石和凹凸棒石对霉菌毒素的吸附作用使其成为重要的吸附剂,然而作为吸附剂的同时不能忽视霉菌毒素的解吸现象,综合考虑吸附与解吸2种现象以便在生产实践中更好的脱除霉菌毒素。The aim of this study was to determine the adsorption and desorption of aflatoxin B1 (AFB1), zearalenone , T-2 toxin to montmorillonite and attapulgite in the artificial gastric juice and intestinal juice. The results showed that the adsorption equilibrium of AFB1 ,zearalenone, T-2 toxin to montmorillonite and attapulgite were established within 1h. The adsorption rates of AFB1 and T-2 toxin to sodium montmorillonite were 96. 8 % ,38 % ,respectively. Zearalenone adsorbed by montmorillonite and attapulgite were less than 10 %. The pH of artificial intestinal fluid had a significant influence on adsorbing mycotoxins (P〈0. 05). The adsorption rate of AFB1 to sodium montmorillonite was larger at pH 5 than pH 3 and pH 7 (P〈0. 05), 90. 1 %. High adsorption efficiency of zearalenone to montmorillonite and attapulgite was achieved at pH 3. The adsorption rate of T-2 toxin to montmorillonite and attapulgite was larger at pH 7 than pH 3 and pH 5 (P〈0. 05). The complex compound formed by montmorillonite or attapulgite with mycotoxins has the desorption phenomena, expecially AFB1 and zearalenone. Because of the adsorption of AFB1 ,zearalenone, T-2 toxin to montmorillonite and attapulgite , they are important adsorbent. However, the desorption phenomena should not be neglected. Selection of montmorillonite or attapulgite as a feed additive should be considered their adsorption and desorption in practice.
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