RP-HPLC法测定香茅油的主要成分  

Determination of main compounds in citronella oil by RP- HPLC

作　　者：赵建芬[1] 刘钊[1] 张颖[1] 

机构地区：[1]肇庆学院化学化工学院,肇庆526061

出　　处：《中国食品添加剂》2014年第7期179-183,共5页China Food Additives

基　　金：肇庆学院自然科学研究项目(201005)

摘　　要：建立同时测定香茅油中香叶醇、柠檬醛、香茅醛、月桂烯的反相高效液相色潽法（RP-HPLC）.色谱柱为Agilent Eclipse XDB-C1s（4.6mm×250mm,5μm）,流动相为乙腈-水（80∶20,v/v）,流速为1.0 mL/min,检测波长为210 nm,柱温为室温,进样量20μL.在此色谱条件下,4组分获得良好的分离且各组分的质量浓度与峰面积呈良好线性关系,相关系数R2为0.9977 ～0.9998.样品中各组分的加标回收率在96.4％ ～99.2％之间,RSD≤2.0％.所建立的HPLC法操作简便,有良好的稳定性及重现性,适用于香茅油中主要成分含量的测定.A method of determining geraniol, citral, citronellal and myrcene in citronella oil simultaneously by re- versed - phase high performance liquid chromatography （ RP - HPLC） was established. Chromatography column was Agilent Eclipse XDB -C18 （4. 6 250 mm, 5 m）, mobile phase was acetonitrile -water （80 ： 20, v/v）, flow rate was 1.0 mL/min, detection wavelength was 210 nm, column temperature was room temperature, and injection volume was 20μL. Under these chromatographic conditions, four analytes were well separated, and a good linear relationship between peak area and concentration was observed for each component. The correlation coefficient R^2 was varied from 0. 9977 to 0. 9998. The average spike recoveries of geraniol, citral and myrcene was form 96.4% to 99. 2%, with RSD ≤2. 0%. This method was simple and had good stability and reproducibility. It was suitable for the determination of these compounds in citronella oil.

关 键 词：香茅油 高效液相色谱 香叶醇 柠檬醛 香茅醛 月桂烯 

分 类 号：Q946.85[生物学—植物学]