表面活性剂辅助提取石榴叶中总黄酮工艺的研究  

Study on extraction of total flavonoids from pomegranate leaf by surfactant

作　　者：宫坤[1,2] 詹小龙[3] 郭中婷 薛建平[2,3] 李进步[2,3] 盛玮[2,3] 

机构地区：[1]淮北普豪生物科技有限公司,安徽濉溪234100 [2]资源植物生物学安徽省重点实验室,安徽淮北235000 [3]淮北师范大学生命科学学院,安徽淮北235000

出　　处：《食品工业科技》2014年第23期261-264,322,共5页Science and Technology of Food Industry

基　　金：安徽省科技计划项目(12070303032);安徽省教育厅自然基金项目(KJ2012A255)

摘　　要：以石榴叶为研究材料,采用单因素和正交实验设计对石榴叶中总黄酮提取工艺进行优化,考察表面活性剂种类及浓度、乙醇浓度、提取温度、提取时间、料液比以及提取次数对石榴叶总黄酮提取率的影响。结果表明:石榴叶总黄酮的最佳提取工艺条件为吐温-40的质量浓度为2.4g/100m L、提取剂乙醇的浓度为40%(v/v)、料液比为1∶30,浸提温度为60℃、提取时间60min。在此条件下提取1次,石榴叶总黄酮的提取率为86.17%。While the pomegranate leaf were used as the research object,the optimum conditions for extraction of total flavonoids from pomegranate leaf were investigated by the single factors and orthogonal array design experiment,and the effect of surfactant kinds, concentration of surfactant and ethanol, extracting temperature, extracting time,liquid-to-material and extracting times on yield of total flavonoids from pomegranate leaf were investigated.The optimum extraction conditions were determined as 2.4g/100mL,40%, 1 ：30 （g/mL）,60℃ and 60min for tween-40 concentration, ethanol concentration, solid/liquid ratio （ g/mL）, temperature and extraction time,respectively.Under the optimized conditions,the extraction rate of total fiavonoids from pomegranate leaf was 86.17% by extracting once.

关 键 词：石榴叶 总黄酮 表面活性剂 

分 类 号：TS201.1[轻工技术与工程—食品科学]