多种提取方法分析蛇莓挥发性组分  

Analysis of Volatile Components in Duchesnea Indica by Different Extraction Methods

作　　者：王晨旭[1] 于兰[2] 杨艳芹[1] 雷永乾[3] 杨卫[1] 徐经伟[1] 

机构地区：[1]中国科学院长春应用化学研究所,长春130022 [2]长春中医药大学第一附属医院,长春130021 [3]中国广州分析测试中心,广州510070

出　　处：《分析化学》2014年第11期1710-1714,共5页Chinese Journal of Analytical Chemistry

摘　　要：采用顶空固相微萃取(HS-SPME)、索氏提取(SE)、超声辅助提取(UAE)、水蒸汽蒸馏法(SD)结合气相-质谱(GC-MS)联用技术分析蛇莓的挥发性组分。实验对HS-SPME各影响因素进行了优化,最终确定采用65μm聚二甲基硅氧烷-二乙烯基苯(PDMS-DVB)萃取头在90℃水浴50 min后插入250℃GC进样口解吸5 min,得到最佳提取效果。HS-SPME,SD,SE和UAE方法分别得到47,32,16和16种挥发性组分,总数为66种,其中47种化合物为首次报道。实验结果表明,HS-SPME与SD得到多为萜类化合物,而SE与UAE得到的化合物中酸类分别占总成分61.44%与69.54%。The volatile compositions of Duchesnea indica were studied by headspace solid-phase microextraction （ HS-SPME ）, soxhlet extraction （ SE ）, ultrasonic assistant extraction （ UAE ） and steam distillation （SD） coupled with gas chromatography-mass spectrometry （GC-MS）. The experimental parameters of HS-SPME, including fiber type, extraction temperature, extraction time and desorption time were investigated. 47, 32, 16 and 16 compounds were identified by HS-SPME, SD, SE and UAE extracting methods, respectively. 66 compounds were obtained in total, among which 47 compounds were first reported in Duchesnea indica. The experimental results showed that terpenoids were the most abundant compositions in HS-SPME and SD, but acids accounted for 61.44% and 69.54% of the total content obtained by SE and UAE.

关 键 词：顶空固相微萃取 气相色谱-质谱联用法 蛇莓 挥发性组分 

分 类 号：O652[理学—分析化学] R284[理学—化学]