小剂量氯胺酮对抑郁大鼠无抽搐电休克抗抑郁治疗起效时间和治疗疗程的影响  

Effect of small-dose ketamine on onset time and course of modified electroconvulsive therapy in mentally depressed rats

作　　者：陈婧[1] 闵苏[1] 罗洁[1] 彭丽桦[1] 律峰[1] 黎平[1] 郝学超[1] 

机构地区：[1]重庆医科大学附属第一医院麻醉科,400016

出　　处：《中华麻醉学杂志》2014年第11期1365-1368,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81271501）;重庆市临床重点学科项目资助（渝卫科教[2007]2号）;卫生部国家临床重点专科建设项目经费资助（财社[2011]170号）

摘　　要：目的 评价小剂量氯胺酮对抑郁大鼠无抽搐电休克抗抑郁治疗起效时间和治疗疗程的影响.方法 选择SPF级成年雄性SD大鼠60只,体重220～250 g,2～3月龄.采用随机数字表法,将其分为6组（n=10）：正常对照组（C组）、抑郁组（D组）、单纯电休克治疗（ECT）组（ECT组）、异丙酚＋ ECT组（PE组）、氯胺酮＋ECT组（KE组）和氯胺酮＋异丙酚＋ECT组（KPE组）.采用慢性不可预见性轻度应激法建立抑郁模型.建模后,C组、D组和ECT组腹腔注射生理盐水8 ml/kg; PE组和KE组分别腹腔注射异丙酚100 mg/kg或氯胺酮10 mg/kg; KPE组腹腔注射氯胺酮10 mg/kg和异丙酚80mg/kg.除C组和D组外,其余各组待大鼠翻正反射消失后行ECT（电流50 mA、频率50 Hz、正弦波、脉冲宽度0.7 ms、持续1 s）,以引起强直-阵挛抽搐发作为处理成功,ECT 1次/d,连续7d.于建模前、建模后1d及每次ECT结束后（T08）行旷场实验,记录水平活动距离与直立次数.于建模后2d和治疗结束后1d行Morris水迷宫实验,记录逃避潜伏期和目标象限停留时间.结果 与C组比较,D组、ECT组、PE组、KE组T1-8时和KPE组L5时水平活动距离与直立次数减少,逃避潜伏期延长,目标象限停留时间缩短（P＜0.05）,KPE组T6.8时水平活动距离、直立次数及治疗后逃避潜伏期与目标象限停留时间差异无统计学意义（P＞0.05）;与D组比较,ECT组和PE组T6-8时、KE组及KPE组T4-8时水平活动距离与直立次数增加,ECT组逃避潜伏期延长,目标象限停留时间缩短,KPE组逃避潜伏期缩短,目标象限停留时间延长（P＜0.05）;与ECT组和PE组比较,KE组T47时、KPE组T4-8时水平活动距离与直立次数增加,KPE组逃避潜伏期缩短,目标象限停留时间延长（P＜0.05）;与KE组比较,KPE组T6-7时水平活动距离与直立次数增加,逃避潜伏期缩短,目标象限停留时间延长（P＜0.05）.结论 小剂量氯胺酮可缩短抑郁大鼠无抽搐电休克抗Objective To evaluate the effect of small-dose ketamine on the onset time and course of modified electroconvulsive therapy （MECT） in mentally depressed rats.Methods Sixty SPF adult male SpragueDawley rats,aged 2-3 months,weighing 220-250 g,were randomly divided into 6 groups （n =10 each） using a random number table：normal control group （group C）,depression group （group D）,ECT group,propofol ＋ ECT group （group PE）,ketamine ＋ ECT group （group KE） and ketamine ＋ propofol ＋ ECT group （group KPE）.The depression model was established by chronic unpredictable mild stress （CUMS）.Mter CUMS,C,D and ECT groups received intraperitoneal normal saline 8 ml/kg,group PE received intraperitoneal propofol 100 ml/kg,group KE received intraperitoneal ketamine 10 ml/kg,and group KPE received intraperitoneal ketamine 10 ml/kg ＋ propofol 80 ml/kg.All the groups received ECT once a day for 7 consecutive days starting from the time point when righting reflex was lost except C and D groups.Open-field test was performed before CUMS,at 1 day after CUMS and at the end of each ECT （T0 8）.The total distance and the number of standing on the back legs were recorded.Morris water maze test was performed at 2 days after CUMS and 1 day after the end of therapy,and the escape latency and time of staying at the original platform quadrant were recorded.Results Compared with group C,the total distance was shortened and the number of standing on the back legs was reduced,the escape latency was prolonged,and the time of staying at the original platform quadrant was shortened at T1-8 in D,ECT,PE and KE groups and at T1 5 in KPE group,and no significant was found in KPE group in the total distance,number of standing on the back legs,escape latency,and time of staying at the original platform quadrant at T6-8.Compared with group D,the total distance was prolonged and the number of standing on the back legs was increased at T6-8 in ECT and PE groups and at T4-8 in KE and KPE groups,the escape latency was prolon

关 键 词：抑郁 氯胺酮 二异丙酚 电惊厥疗法 

分 类 号：R749.4[医药卫生—神经病学与精神病学]