结直肠癌K-ras基因突变检测及其意义  

Detection on K-ras gene in colorectal cancer and its significance

作　　者：张博淼[1] 迟强[1] 李大川[1] 陈海鹏[1] 张恒春[1] 王锡山[1] 

机构地区：[1]哈尔滨医科大学附属第二医院普外科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2014年第5期395-398,共4页Journal of Harbin Medical University

摘　　要：目的研究结直肠癌中K-ras基因突变率及突变类型,探讨结直肠癌K-ras基因野生型及突变型与患者临床参数的关系;通过基因测序,指导临床靶向药物用药。方法随机选取哈尔滨医科大学附属第三医院结直肠外科2008年9月至2010年3月收治的100例结直肠癌患者组织标本通过手术室取材、组织标本DNA提取、逆转录PCR、凝胶电泳、胶回收、直接测序等技术筛选K-ras基因突变标本进行统计分析,从中得出突变率及突变类型。结果共筛选出了34例(34%)K-ras基因突变标本,其基因突变均发生在第一外显子的第12及第13密码子。结果表明:第一外显子的第12密码子GGT突变为GAT或GTT;第一外显子第13密码子由GGC突变为GGT。其中并未发现第二外显子第59及第61位发生突变,及第一外显子12位13位同时突变。结论 K-ras基因结直肠癌中约有34%发生突变。在K-ras基因突变类型与患者的病期、病理类型、肿瘤的浸润深度、发病部位等因素无关。Objective To study the K-ras gene mutation rate and its types of mutation in color-ectal carcinoma.To correlate wild type and mutation type of K-ras gene with clinicopathologic parameters.Through gene sequencing it to guide clinical application of targeted drugs.Meth-ods One hundred samples of colorectal cancer which tissues were collected from the Third Af-filiated Hospital of Harbin Medical University from September 2008 to March 2010.Tissue DNA extraction, real-time quantitative polymerase chain （ PCR） , gel electrophoresis, gel recy-cling, direct sequencing screening of k-ras gene mutation specimens, and statistic analysis were adopted to find the mutation rate and mutation characteristics.Results 34 k-ras gene mutation cases were screened out （34%） in specimens, which all occurred in the first exon of the 12th and 13th.The results showed that the first exon of codon 12th GGT mutated into GAT or GTT, and 13th the first exon of codon GGC mutated into GGT.However, no mutations in the second exon of the second of the 59th or in the third exon of the 61th were found in these specimens. Conclusion About 34%of k-ras gene mutates in colorectal carcinoma patients.The types of k-ras gene mutation are not related to clinicopathologic parameters.

关 键 词：K—ras 西妥昔单抗 结直肠癌 基因测序 

分 类 号：R735.3[医药卫生—肿瘤]