正交实验优化超声辅助提取牡丹种皮总黄酮及其抗凝血活性研究  

Ultrasonic-assisted Extraction of Total Flavonoids from Peony Tree Seed Coat and Its Anticoagulation Activity

作　　者：王洪政[1,2] 刘伟 张琳[1,2] 丛赢[1,2] 昝鹏[1,2] 王化[1,2,3] 祖元刚[1,2] 陈华峰[1,2] 赵修华[1,2] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040 [2]生物资源生态利用国家地方联合工程实验室,哈尔滨150040 [3]黑龙江省科学院自然与生态研究所,哈尔滨150040

出　　处：《植物研究》2014年第6期856-860,共5页Bulletin of Botanical Research

基　　金：林业科学技术研究项目(2013-01);中央高校基本科研业务费专项资金项目资助(2572014CA09)

摘　　要：采用超声辅助法提取牡丹种皮黄酮,通过乙醇体积分数、液固体积质量比(mL·g^(-1))、超声时间和超声功率4个因素对牡丹种皮黄酮提取得率的影响进行了正交实验,确定超声辅助提取牡丹种皮黄酮最佳条件为:乙醇体积分数为55%,液固体积质量比为20:1,超声提取时间为40 min,超声功率为140 W,牡丹种皮黄酮得率为46.50‰。经过柱层析纯化后,牡丹种皮黄酮中含有203.5mg·g^(-1)槲皮素和23.2mg·g^(-1)木犀草素。大鼠体内实验证明牡丹种皮黄酮具有良好的抗凝血作用。We used ultrasonic-assisted extraction method to extract total flavonoids from seed coat of peony tree,and studied the extraction conditions including volume fraction of ethanol,ratio of liquid to solid（mL · g^-1）,ultrasonic time and power which influenced the extraction yield of total flavonoids.The results of orthogonal test indicated the optimum extraction conditions of total flavonoids of seed coat were;volume fraction of 55%as solvent,liquid to solid ratio of 20：1,ultrasonic time of 40 min and ultrasonic power of 140 W.Under these conditions,the total flavonoids yield of seed coat was 46.50‰.After column chromatography for flavonoids purification of seed coat extract,it contained 203.5 mg · g^-1 quercetin and 23.2 mg · g^-1 luteohn.Seed coat flavonoids of peony tree showed high activity of anticoagulation activity in experiments of in vivo rat.

关 键 词：超声辅助提取 牡丹种皮 黄酮 抗凝血 

分 类 号：TS236.9[轻工技术与工程—粮食、油脂及植物蛋白工程]