Gastrodin prevents steroid-induced osteonecrosis of the femoral head in rats by anti-apoptosis  

作　　者：Zheng Huifeng Yang Erping Peng Hao Li Jianping Chen Sen Zhou Jianlin Fang Hongsong Qiu Bo Wang Zhe 

机构地区：[1]Department of Orthopedics, Renmin Hospital of Wuhan University,Wuhan, Hubei 430060, China

出　　处：《Chinese Medical Journal》2014年第22期3926-3931,共6页中华医学杂志（英文版）

基　　金：This work was supported by a grant from the National Natural Science Foundation of China （No. 81301592）.

摘　　要：Background Gastrodin,as one of the major components extracted from the Chinese herb Gastrodia elata BI.,has many biologic effects,one of which is anti-apoptosis.Apoptosis is considered to be one of the pathogenetic mechanisms in steroid-induced osteonecrosis of the femoral head （ONFH）.Therefore,we performed this study to investigate whether gastrodin has the potential to prevent steroid-induced ONFH.Methods All 18 male adult Wistar rats were divided equally into three groups：the steroid group,the gastrodin＋steroid group,and the control group.Osteonecrosis was induced by low-dose lipopolysaccharide and subsequent high-dose methylprednisolone.Histomorphometric method was used to determine the incidence of osteonecrosis.Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling （TUNEL） assay was performed to detect apoptotic index of osteocytes and osteoblasts.Real-time PCR and Western blotting were performed to detect mRNA and protein expression of Bax,Bcl-2,and Caspase-3.Fisher＇s exact probability test and one-way analysis of variance （ANOVA） with Turkey＇s post hoc test were used to examine significant differences between groups.Results The incidence of osteonecrosis in the gastrodin＋steroid group （16.7％） was significantly lower than that in the steroid group （83.3％）.According to TUNEL assay,the apoptotic indices in the steroid group,the gastrodin＋steroid group,and the control group were 91.1％,27.1％,and 5.4％,respectively,and the differences were significant between groups.Compared with the control group and the gastrodin＋steroid group,the mRNA and protein expression levels of Bax and Caspase-3 were significantly higher in the steroid group,but the Bcl-2 mRNA and protein expression levels were significantly lower.Conclusion Gastrodin could prevent steroid-induced ONFH by anti-apoptosis.Background Gastrodin,as one of the major components extracted from the Chinese herb Gastrodia elata BI.,has many biologic effects,one of which is anti-apoptosis.Apoptosis is considered to be one of the pathogenetic mechanisms in steroid-induced osteonecrosis of the femoral head （ONFH）.Therefore,we performed this study to investigate whether gastrodin has the potential to prevent steroid-induced ONFH.Methods All 18 male adult Wistar rats were divided equally into three groups：the steroid group,the gastrodin＋steroid group,and the control group.Osteonecrosis was induced by low-dose lipopolysaccharide and subsequent high-dose methylprednisolone.Histomorphometric method was used to determine the incidence of osteonecrosis.Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling （TUNEL） assay was performed to detect apoptotic index of osteocytes and osteoblasts.Real-time PCR and Western blotting were performed to detect mRNA and protein expression of Bax,Bcl-2,and Caspase-3.Fisher＇s exact probability test and one-way analysis of variance （ANOVA） with Turkey＇s post hoc test were used to examine significant differences between groups.Results The incidence of osteonecrosis in the gastrodin＋steroid group （16.7％） was significantly lower than that in the steroid group （83.3％）.According to TUNEL assay,the apoptotic indices in the steroid group,the gastrodin＋steroid group,and the control group were 91.1％,27.1％,and 5.4％,respectively,and the differences were significant between groups.Compared with the control group and the gastrodin＋steroid group,the mRNA and protein expression levels of Bax and Caspase-3 were significantly higher in the steroid group,but the Bcl-2 mRNA and protein expression levels were significantly lower.Conclusion Gastrodin could prevent steroid-induced ONFH by anti-apoptosis.

关 键 词：GLUCOCORTICOIDS GASTRODIN femoral head osteonecrosis PREVENTION APOPTOSIS 

分 类 号：Q959.837[生物学—动物学] S858.31[农业科学—临床兽医学]