叶片乙醇酸氧化酶基因全长cDNA的分离与表达研究  

Isolation of Full-length cDNA of GLO from Pranus salicina Leaf and its Expression

作　　者：赵利[1,2] 陈桂信[1,2] 潘东明[1,2] 王玉珍[1,2] 吕恃衡[1] 姜翠翠[3] 

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学园艺产品贮运保鲜研究所,福建福州350002 [3]福建省农业科学院果树研究所,福建福州350013

出　　处：《江西农业大学学报》2014年第5期965-970,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：科技部国家科技支撑计划项目(2007BAD07B01);福建省种业创新与产业化工程项目和福建农林大学创新团队项目(cxtd12013)

摘　　要：乙醇酸氧化酶(glycolate oxidase,GLO)是植物光呼吸途径中的一种限速酶,催化羟乙酸盐氧化成乙醛酸盐和H2O2,与植物的诱导抗病性密切相关。试验从已构建好的(Prunus salicina Lindl.var.cordata J.Y.Zhang et al.)5个不同生长发育时期叶片的均一化全长cDNA文库中分离得到了一个乙醇酸氧化酶(GLO)基因,命名为PsGLO。序列分析表明,PsGLO基因cDNA全长为1 531 bp,开放阅读框为1 122 bp,编码374个氨基酸。氨基酸多重序列比对表明,该基因与陆地棉乙醇酸氧化酶基因相似性最高,为90%。荧光定量PCR分析表明,PsGLO基因在叶中木奈叶芽、展开叶、幼叶、成熟叶、老叶5个不同生长发育时期中都有表达,其中,老叶中表达量最高,叶芽最低。Glycolate oxidase （ GLO） is a key enzyme in photorespiration and catalyzes the oxidation of gly-colate into glyoxylate with an equimolar amount of H2 O2 ,which is related with induction of disease resistance in plant.A clone,named PsGLO,was separated from cDNA library prepared from five different growth and de-velopment periods of leaf of Prunus salicina.Sequence analysis showed that PsGLO was 1 531 bp in length with a 1 122 nucleotides ORF that putatively encoded a protein with 374 amino acids.Multiple sequence alignments and phylogenetic tress analyses showde that GLO was clustered with Gossypium hirsutum GLO and showed high similarity （90%） in amino acid sequences.Quantitative real-time PCR analysis showed that the expression of PsGLO was observed in all different stages tested here including leafbud,unfoldedleaf,spire,climax leaf,old leaf and the highest expression level was found in old leaves,but the lowest in leafbud.

关 键 词：木奈(Prunus salicina Lindl.var.cordata J.Y.Zhang et al.) 乙醇酸氧化酶 全长CDNA 表达分析 

分 类 号：S662.3[农业科学—果树学]