高效液相色谱法同时测定果冻中9种添加剂  

Determination of 9 Additives in Jelly by High Performance Liquid Chromatography

作　　者：钱聪[1] 

机构地区：[1]国家食品质量安全监督检验中心,北京100094

出　　处：《食品研究与开发》2014年第21期109-111,共3页Food Research and Development

摘　　要：建立了果冻中9种添加剂的高效液相色谱测定法。采用ZORBAX Eclipse XDB-C18,以甲醇和乙酸铵为流动相,梯度洗脱,流速为1.0 m L/min,检测波长为230、415、515、630 nm,柱温30℃,采用高效液相色谱DAD检测器进行测定。该方法的检出限介于0.25 mg/L^0.5 mg/L之间,加标回收率95.6%~114.0%,相对标准偏差为1.09%~4.28%(n=4)。This thesis developed a HPLC method for determination of 9 additives in Jelly,which were separated on ZORBAX Eclipse XDB-C18 using cabinol and amoniumacetate as the mobil phase at a flow rate of 1.0 mL/min, with the column tenperate 30℃and detection wave 230, 415, 515, 630 nm by liquid chromatography with DAD detector. The limit of quantitation are between 0.25 and 0.5 mg/kg.Overall recoveries were between 95.6%and 114.0%, and RSD （n=4）value were 1.09%and 4.28%.

关 键 词：高效液相色谱仪 果冻 添加剂 

分 类 号：O657.72[理学—分析化学]