复合酶制剂羊饲料添加剂中纤维素酶和淀粉酶影响因素的研究  

Influence factors of cellulase and amylase of compound enzyme preparation in sheep feed additive

作　　者：李忠玲[1] 徐升运[1] 李文孝[1] 李本光[2] 岳淑宁[2] 张红艳[1] 赵文娟[2] 付博[2] 

机构地区：[1]陕西省科学院酶工程研究所,陕西西安710600 [2]陕西省酶工程技术中心,陕西西安710600

出　　处：《中国酿造》2014年第11期85-89,共5页China Brewing

基　　金：陕西省科学院应用基础项目(2012K-15);陕西省科技统筹创新工程难题攻关项目(2013KTZB02-02-02)

摘　　要：研究了蛋白酶对纤维素酶、淀粉酶活力的影响,结果表明淀粉酶对蛋白酶比较敏感,淀粉酶在质量浓度为150 U/m L的蛋白酶条件下37℃处理30 min,相对酶活力为75.79%。研究了纤维素酶和淀粉酶的热稳定性,结果表明与纤维素酶相比,淀粉酶对高温更敏感,淀粉酶在100℃的沸水中处理1 min,相对酶活力为1.89%。研究了金属离子对纤维素酶和淀粉酶的影响,结果表明,对于纤维素酶激活作用大小顺序依次为Cu2+>Zn2+>Co2+>Mn2+>Fe2+,抑制作用大小顺序依次为Mn2+>Fe2+>Co2+>Zn2+>Cu2+;对于淀粉酶激活作用不明显,抑制作用大小顺序依次为Fe2+>Cu2+>Mn2+>Zn2+>Co2+。以上研究的目的是为酶在饲料中的应用提供参考。The effectofprotease on the activity ofcellulase and am ylase w as studied,and resultshow ed thatam ylase w as m ore sensitive to protease than cellulase.The relative enzym atic activity ofam ylase w as75.79% w hen treated in 150 u/m lprotease at37 ℃ for30 m in.The therm ostabilitiesof cellulase and am ylase w ere tested and the resultsshow ed thatam ylase w asm ore sensitive to high tem perature than cellulase.The relative enzym e ac-tivity ofam ylase w as1.89% w hen treated in 100 ℃ w aterfor1 m in.Effectsofdifferentm etalionson the activity ofcellulase and am ylase w ere test-ed and the results show ed thatactivation effecton cellulase ranked in order w as C u^2＋,Zn^2＋,C o^2＋,M n^2＋ and Fe^2＋,the inhibition effecton cellulase ranked in orderw asM n^2＋,Fe^2＋,C o^2＋,Z^n2＋ and C u^2＋.A ctivation effecton am ylase w asnotdistinctand inhibition effecton am ylase ranked in orderw as Fe^2＋,C u^2＋,M n^2＋,Zn^2＋ and C o^2＋.The research purpose w asto provide a convenientreference forfurtherresearch aboutenzym e applicationsto feed.

关 键 词：纤维素酶 淀粉酶 酶制剂 饲料添加剂 

分 类 号：S816.7[农业科学—饲料科学]