黄参籽多酚的组成及其对人结肠癌细胞Lovo的抑制作用  

Polyphenolic Composition of Sphallerocarpus gracilis Seeds and Their Inhibitory Effect on Human Colon Carcinoma (Lovo) Cells

作　　者：高春燕[1] 田呈瑞[2] 卢跃红[3] 

机构地区：[1]大理学院公共卫生学院,云南大理671003 [2]陕西师范大学食品工程与营养科学学院,陕西西安710062 [3]大理学院农学与生物科学学院,云南大理671003

出　　处：《现代食品科技》2014年第11期1-5,11,共6页Modern Food Science and Technology

摘　　要：采用超声波辅助提取技术从黄参籽中提取了游离酚和不溶性细胞壁结合酚,并采用福林-酚法测定了其多酚含量。同时,采用HPLC-MS对黄参籽多酚的组成进行了分析,并采用MTT法测定了其对人结肠癌细胞Lovo的抑制作用。结果显示,黄参籽中主要的酚类化合物是绿原酸、咖啡酸、木犀草素葡萄糖甙、芍药花素二葡萄糖甙、二咖啡酰奎尼酸葡萄糖甙和儿茶酸葡萄糖甙,且不同的酚类化合物在游离酚和不溶性细胞壁结合酚提取物中的含量存在差异;黄参籽多酚提取物对人结肠癌细胞的增殖具有显著的抑制作,且随着多酚浓度的增加,抑制率上升,呈剂量效应关系;形态学的观察结果表明,经黄参籽多酚提取物处理后细胞形态发生明显改变,细胞固缩、干瘪,体积变小,甚至干枯脱落,初步提示黄参籽多酚可以抑制人结肠癌细胞Lovo的生长。The free and insoluble cell wall-bound phenolics of Sphallerocarpus gracilis seeds were characterized by ultrasound-assisted extraction. The polyphenolic contents were determined by the Folin-Ciocalteu method and high-performance liquid chromatography-mass spectrometry （I-IPLC-MS）, and the inhibitory effects of the polyphenolic extracts on human colon carcinoma （Lovo） cells were determined by an MTT assay. The results indicated that the dominant phenolic compounds in S. gracilis seeds were chlorogenic acid, caffeic acid, paeoniflorin-diglucoside, luteolin-glucoside, dicaffeoylquinic acid glucoside, and protocatechuic acid glucoside, and that the free and insoluble cell-wall-bound phenolic content in extracts differed. The phenolic extracts from S. gracilis seeds showed significant inhibitory effects on Lovo cells. Furthermore, the inhibition rate gradually enhanced with increasing phenolic concentrations, showing a dose-dependent effect. The morphology of cells changed substantially after treatment with polyphenolic extracts; cells were smaller, shriveled, and even dried and detached, preliminarily suggesting that polyphenolic extracts from S. gracilis seeds could inhibit the growth of Lovo cells.

关 键 词：黄参籽 多酚 抗肿瘤活性 人结肠癌细胞 

分 类 号：R285[医药卫生—中药学]