上海大气降水中细菌气溶胶的多样性研究  

Diversity of Bacterial Areasols in Precipitation of Shanghai Area

作　　者：梁宗敏[1] 杜睿[1] 杜鹏瑞 王亚玲[1] 李梓铭[1] 

机构地区：[1]中国科学院大学资源与环境学院,北京100049

出　　处：《环境科学》2014年第7期2483-2489,共7页Environmental Science

基　　金：国家自然科学基金项目(41175135)

摘　　要：采用分子生物学技术16S rRNA克隆文库方法,以上海市2012年不同月份的大气降水样品为模板构建细菌16S rRNA克隆文库,对连续5个月的大气降水样品中细菌群落结构组成及多样性进行了研究,并对其群落结构的生物多样性、物种丰度及覆盖率等进行了分析比较.结果表明,变形菌门（Proteobacteria）（α-,β-,γ-）是上海市降水样品中细菌的优势菌群（32.5%-94.1%）,另外还包括拟杆菌门（Bacteroidetes）、放线菌门（Actinobacteria）、异常球菌-栖热菌门（Deinococcus-Thermus）、蓝藻门（Cyanobacteria）、酸杆菌门（Acidobacteria）、厚壁菌门（Firmicutes）共7个主要门类的细菌,以及未定菌（TM7）.多样性指数分析结果显示,不同月份降水样品的细菌群落结构组成与多样性均存在着差异性,夏季6月样品的细菌群落生物多样性及物种丰度明显高于其它样品,其次是秋季8月的样品.In the present study,16S rRNA gene sequencing based approaches were used to analyze the bacterial communities and diversity in the precipitation samples from Shanghai city,which were carried out in different months in 2012. Five clone libraries were established based on the rainwater samples collected during 5 different precipitation periods. The results showed that Proteobacteria（ α-,β-,γ-）（ 32.5%-94.1%） were dominant in the precipitation,including Alphaproteobacteria,Betaproteobacteria,and Gammaproteobacteria, while other 6 bacterial communities, such as Bacteroidetes, Actinobacteria, Deinococcus-Thermus,Cyanobacteria,Acidobacteria,and Firmicutes,were involved as well. Furthermore,a small proportion of undetermined bacteria（ TM7,2.5%） were also found. In addition,microbial diversity in the sample of SH6 was obviously more than those in other samplers. The investigation revealed that there was a difference in the bacterial communities and diversity in precipitation of different months.

关 键 词：降水 细菌 群落结构 多样性 16S RRNA 

分 类 号：X172[环境科学与工程—环境科学] X513