高通量测序法检测胃癌患者融合基因LRP5-LITAF  

Determination of LRP5-LITAF fusion gene in gastric cancer by transcriptome analysis

作　　者：徐晓凤[1] 王建江[2] 叶金松[1] 徐丽芳[3] 朱蕴玲[1] 陶岚[1] 薛迎春[2] 

机构地区：[1]靖江市人民医院检验科,江苏靖江214500 [2]靖江市人民医院普外科,江苏靖江214500 [3]靖江市人民医院消化科,江苏靖江214500

出　　处：《临床检验杂志》2014年第11期828-830,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：目的了解胃癌发病过程中融合基因表达水平的变化,探讨其在胃癌的发病机制中的作用。方法用Illumina高通量测序仪对早期胃癌患者组织及匹配的癌旁组织进行RNA测序,以检测差异基因表达水平及结构变化。结果在胃癌组织中发现1 590个基因上调和709个基因下调;功能富集分析表明,这些差异表达基因富集于细胞周期、肿瘤侵入与扩散有关的基因实体(gene ontology,GO)注释中;且在约40%(2/5)的胃癌患者的癌组织中发现融合基因LRP5-LITAF。结论发现了胃癌发病过程中的大量差异表达基因及融合基因LRP5-LITAF,为胃癌的辅助诊断及靶向治疗提供了初步实验依据。Objective To investigate the change of fusion gene levels in the development of gastric cancer, explore the possible patho- genesy of gastric cancer. Methods RNAs in tumor tissues and matched normal tissues in the patients with gastric cancer were per- formed sequencing using Illumina high-throughput sequencer, and the levels and structural variations of differentially expressed genes were analyzed. Results One thousand five hundred and ninety up-regulated and 709 down-regulated genes were detected in gastric tumor tissues. These differentially expressed genes were enriched in gene ontology （GO） related to cell cycle and tumor invasion and proliferation. Moreover, LRP5-LITAF fusion gene was found in 40% （2/5） of gastric tumor tissues. Conclusion Many differentially expressed genes and LRPb-LITAF fusion gene were identified in gastric tumor tissues, which provided the foundation for the diagnosis and therapy of gastric cancer.

关 键 词：胃癌 转录组 融合基因 LRP5-LITAF 高通量测序 

分 类 号：R735.2[医药卫生—肿瘤]